Calnexin Antibody (IE2.1C12)

Western Blot: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Calnexin (1E2.1C12) antibody was tested in Hela cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Flow Cytometry: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Flow (Intracellular): Calnexin Antibody (IE2.1C12) [NBP2-36571] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: IE2.1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Calnexin Antibody (IE2.1C12) Summary

• Immunogen: Partial recombinant human Calnexin protein (between amino acids 1-300) [UniProt# P27824]
• Localization: Endoplasmic reticulum membrane, Melanosome
• Marker: Endoplasmic Reticulum Membrane Marker
• Predicted Species: Mouse (94%), Rat (94%), Primate (99%), Canine (95%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CANX
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 7 ug/ml
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Flow (Intracellular) 5 ug/million cells
• Control:

  Recombinant Human Calnexin Protein
  Calnexin Overexpression Lysate
  Calnexin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Calnexin Antibody (IE2.1C12)

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calnexin Antibody (NBP2-36571)(2)

Publications using NBP2-36571

• Munson PB, Hall EM, Farina NH et al. Exosomal miR-16-5p as a target for malignant mesothelioma Sci Rep Aug 12 2019 [PMID: 31406207] (WB, Human)
• MacPherson Maximilian, Westbom Catherine, Kogan Helen, Shukla Arti Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro. Histochem Cell Biol. 2016 Dec 24 [PMID: 28013367] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 821(Human)
  • 12330(Mouse)
  • 29144(Rat)
• Uniprot:
  • P27824(Human)