Calnexin Antibody (CANX/9147R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/9147R) - Azide and BSA Free [NBP3-24216] - Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Calnexin Antibody (CANX/9147R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: CANX/9147R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Calnexin Antibody (CANX/9147R) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
• Localization: Cytoplasm.
• Marker: Endoplasmic Reticulum Marker
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CANX
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Western Blot 1-2ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order antibody without BSA.
  
  Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calnexin Antibody (CANX/9147R) - Azide and BSA Free

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CANX