Calnexin Antibody (1C2.2D11) - BSA Free

Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin [1C2.2D11] Antibody NBP2-36570AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Calnexin (1C2.2D11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Calnexin (IC2.2D11) was detected in immersion U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (IC2.2D11) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-36570AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Calnexin (1C2.2D11) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (1C2.2D11) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NBP2-36570F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Rt, Bv, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 1C2.2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Calnexin Antibody (1C2.2D11) - BSA Free Summary

• Description: Novus Biologicals Mouse Calnexin Antibody (1C2.2D11) - BSA Free (NBP2-36570) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Calnexin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partial recombinant human Calnexin protein (between amino acids 1-300). [UniProt P27824]
• Localization: Endoplasmic reticulum membrane, Melanosome
• Marker: Endoplasmic Reticulum Membrane Marker
• Predicted Species: Rat (90%), Canine (92%), Primate (99%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CANX
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 5 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 7 ug/ml
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Western Blot
• Control:

  Recombinant Human Calnexin Protein
  Calnexin Overexpression Lysate
  Calnexin Overexpression Lysate

Reactivity Notes

Immunogen's sequence similarity with other species: Mouse (88%), Duck (81%), Xenopus (76%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Calnexin Antibody (1C2.2D11) - BSA Free

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calnexin Antibody (NBP2-36570)(2)

Publications using NBP2-36570

• Megan AP, Rebecca K, Claudia B et al. RNAi-based ALOX15B silencing augments keratinocyte inflammation in vitro via EGFR/STAT1/JAK1 signalling Cell Death Dis. 2025-01-22 [PMID: 39843435] (Immunocytochemistry/ Immunofluorescence, Human)
• Ma A, Motyka B, Gutfreund K et al. A Dendritic Cell Receptor-Targeted Chimeric Immunotherapeutic Protein (C-HBV) for the Treatment of Chronic Hepatitis B Hum Vaccin Immunother. 2019-12-12 [PMID: 31687879] (Human)
  
  Details:
  Citation used the FITC format of this antibody.

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 821(Human)
  • 12330(Mouse)
  • 29144(Rat)
• Uniprot:
  • P27824(Human)