Caldesmon/CALD1 Antibody (CALD1/820 + h-CALD)

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (CALD1/820 + h-CALD) [NBP2-44424] - Human Uterus stained with Caldesmon Monoclonal Antibody (CALD1/820 + h-CALD).

• Reactivity: Hu
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: CALD1/820 + h-CALD
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Caldesmon/CALD1 Antibody (CALD1/820 + h-CALD) Summary

• Immunogen: Recombinant full-length human CALD1 protein (CALD1/820); Crude human uterus extract (h-CALD)
• Localization: Cytoplasmic
• Marker: Smooth Muscle Marker
• Specificity: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 7q33
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Caldesmon/CALD1 Antibody (CALD1/820 + h-CALD)

• CAD
• CALD1
• caldesmon 1
• Caldesmon
• CDM
• CDMH-CAD
• HCAD
• LCAD
• L-CAD
• MGC21352
• NAG22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALD1
• Uniprot:
  • Q05682(Human)