Calcium-sensing R/CaSR Antibody

Product Discontinued

Calcium-sensing R/CaSR Antibody Summary

• Immunogen: Synthetic 17 amino acid peptide from N-terminal extracellular domain of human CASR.
• Localization: Integral membrane protein.
• Specificity: Human CASR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Opossum (100%), Xenopus (94%), Turkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: ICC/IF usage is reported in scientific literature (PMID: 24217682).
• Control:

  Calcium-sensing R/CaSR Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calcium-sensing R/CaSR Antibody

• calcium-sensing receptor
• CAR
• CaSR
• EIG8
• extracellular calcium-sensing receptor
• FHH
• FIH
• GPRC2A
• GPRC2AHHC
• HHC1
• hypocalciuric hypercalcemia 1
• NSHPT
• parathyroid Ca(2+)-sensing receptor 1
• Parathyroid cell calcium-sensing receptor
• PCaR1
• PCAR1MGC138441

Background

CASR, a Calcium-Sensing Receptor, functions as a sensor in parathyroid and kidney to determine extracellular calcium concentration and to maintain calcium homeostasis. This receptor regulates both secretion of parathyroid hormone and excretion of urinary calcium. Mutations that inactivate CASR cause familial hypocalciuric hypercalcemia, which is also correlated with hyperparathyroidism. On the other hand, mutations that activate CASR cause autosomal dominant hypocalcemia. Hjalm et al. (2001) and Awata et al. (2001) have identified the binding of filamin-1 to the C-terminal tail of CASR, which may contribute to its localization in caveolae, link it to the actin-based cytoskeleton, and participate in CASR-mediated activation of MAPK. Multiple isoforms for CASR are produced by alternative splicing. CASR has been documented in bone marrow, brain, breast (mostly cancer), colon, kidney, parathyroid, skin, stomach, and thyroid. ESTs have been isolated from normal kidney and gallbladder, in addition to parathyroid cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcium-sensing R/CaSR Antibody (NLS1425)(1)

Publication using NLS1425

• Henaut L, Boudot C, Massy ZA et al. Calcimimetics increase CaSR expression and reduce mineralization in vascular smooth muscle cells: mechanisms of action. Cardiovasc Res. 2013-11-28 [PMID: 24217682] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CASR
• Entrez:
  • 846(Human)