CADPS Antibody

Images

 
Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: CADPS Antibody [NBP1-77323] - Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CADPS Antibody [NBP1-77323] - Human brain with CADPS antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CADPS Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human CAPS1. The immunogen is located within the last 50 amino acids of CAPS1.
Specificity
Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CADPS
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Control Peptide
CADPS Peptide (NBP1-77323PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CADPS Antibody

  • Ca++-dependent secretion activator
  • CADPS1
  • Calcium-dependent activator protein for secretion 1
  • calcium-dependent secretion activator 1
  • CAPS-1
  • CAPS1Ca2+-dependent secretion activator
  • CAPSCa2+-dependent activator protein for secretion
  • KIAA1121Ca2+-regulated cytoskeletal protein

Background

CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4,5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion. Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CADPS
Uniprot