CADPS Antibody - BSA Free

Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: CADPS Antibody - BSA Free [NBP1-77323] - Immunofluorescence of CADPS in human brain tissue with CADPS antibody at 20 u/mL.

Immunohistochemistry: CADPS Antibody - BSA Free [NBP1-77323] - Immunohistochemistry of CADPS in human brain with CADPS antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CADPS Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CADPS Antibody - BSA Free (NBP1-77323) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-CADPS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human CAPS1. The immunogen is located within the last 50 amino acids of CAPS1. Amino Acid Squence: DSTLNSKTYETIRNRLTVEE
• Specificity: Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CADPS
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  CADPS Peptide (NBP1-77323PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CADPS Antibody - BSA Free

• Ca++-dependent secretion activator
• CADPS1
• Calcium-dependent activator protein for secretion 1
• calcium-dependent secretion activator 1
• CAPS-1
• CAPS1Ca2+-dependent secretion activator
• CAPSCa2+-dependent activator protein for secretion
• KIAA1121Ca2+-regulated cytoskeletal protein

Background

CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4,5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion. Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CADPS Antibody (NBP1-77323)(1)

Publication using NBP1-77323

• Huisman BD, Michelson DA, Rubin SA, Kohlsaat K et Al. Cross-species analyses of thymic mimetic cells reveal evolutionarily ancient origins and both conserved and species-specific elements Immunity 2024-12-28 [PMID: 39731911]

Bioinformatics

• Gene Symbol: CADPS
• Uniprot:
  • NP_899631(Human)