Cadherin-6/KCAD Antibody

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Western Blot: Cadherin-6/KCAD Antibody [NBP2-17011] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cadherin-6/KCAD Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human K-cadherin. The exact sequence is proprietary.
Localization
Cell membrane
Predicted Species
Mouse (97%), Rat (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDH6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-17011 in the following application:

Reactivity Notes

Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cadherin-6/KCAD Antibody

  • cadherin 6, type 2, K-cadherin (fetal kidney)
  • cadherin, fetal kidney
  • Cadherin6
  • Cadherin-6
  • CDH6
  • KCAD
  • K-cadherin (fetal kidney)
  • K-Cadherin

Background

This gene encodes a type II classical cadherin from the cadherin superfamily. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of this gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cadherin-6/KCAD Antibody (NBP2-17011) (0)

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Review for Cadherin-6/KCAD Antibody (NBP2-17011) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-17011:
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WB
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Images Ratings Applications Species Date Details
Western Blot Cadherin-6/KCAD NBP2-17011
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reviewed by:
Anonymous
WB Human 10/07/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman cancer cell lysate
SpeciesHuman
Lot40849

Blocking

Blocking Details5% non-fat milk in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 in 5% non-fat milk in TBS buffer overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:3000

Details

Detection Notes1 min exposure show strong band.

Comments

CommentsI am not 100% sure if the band show up is Cadherin-6 since the molecular weight is off. The band is very strong in T47D cells, but no signal in MCF7 cells. More experiments are needed to confirm the result.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cadherin-6/KCAD Antibody (NBP2-17011) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cadherin-6/KCAD Antibody (NBP2-17011)

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Diseases for Cadherin-6/KCAD Antibody (NBP2-17011)

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Recent Reviews

4
5
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4
1
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0
2
0
1
0

Anonymous
10/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDH6
Entrez
Uniprot