Recombinant Human Cadherin-8 (CAD-8) Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Cadherin-8 (CAD-8) Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. When 5 x 104 cells/well are added to Recombinant Human Cadherin‑8 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately >30% will adhere after 30 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Cadherin-8 protein
Human Cadherin-8
(Ala23-Met614)
Accession # AAA35628
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CDH8
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
93 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
116-130 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cadherin-8 (CAD-8) Fc Chimera Protein, CF

  • cadherin 8, type 2
  • Cadherin8
  • Cadherin-8
  • CDH8
  • Nbla04261
  • putative protein product of Nbla04261

Background

Cadherin-8 is a member of the type II, also called atypical, subfamily of classic cadherin cell adhesion molecules. Cadherins are transmembrane calcium-dependent cell adhesion proteins. On their cytoplasmic side, they associate with the three catenins, alpha, beta and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Type I cadherins consist of a large extracellular domain with an N-terminal propeptide sequence that is proteolytically cleaved intracellularly, five cadherin repeats and four calcium-binding pockets between the cadherin repeats a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Within the N-terminal cadherin domain, a conserved HAV motif involved in homophilic interaction is present. In contrast, the type II cadherins do not contain the HAV motif in the N-terminal cadherin domain and display weak or no cell adhesive properties. There also appears to be more diversity in the cytoplasmic domains of the type II cadherins as compared to the type I cadherins. Cadherin-8 is most highly expressed in neuronal tissues. The rat Cadherin-8 protein has been suggested to play a role in long-term potentiation. Human Cadherin-8 is a 799 amino acid (aa) residue protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 560 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.

  1. Tanihara, H. et al. (1994) Cell Adhes. Comm. 2:15.
  2. Suzuki, S. et al. (1991) Cell Regul. 2:261.
  3. Nollet, F. et al. (2000) J. Mol. Biol. 299:551.
  4. Yamagata, K. et al. (1999) J. Biol. Chem. 274:19473.
  5. Kido, M. et al. (1998) Genomics 48:186.

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Bioinformatics

Gene Symbol CDH8
Entrez
Uniprot