Cadherin-18 Antibody (6F7) - Azide and BSA Free

Western Blot: Cadherin-18 Antibody (6F7) [H00001016-M01] - CDH18 monoclonal antibody (M01), clone 6F7 Analysis of CDH18 expression in HeLa.

Immunohistochemistry-Paraffin: Cadherin-18 Antibody (6F7) [H00001016-M01] - Analysis of monoclonal antibody to CDH18 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Sandwich ELISA: Cadherin-18 Antibody (6F7) [H00001016-M01] - Detection limit for recombinant GST tagged CDH18 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 6F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cadherin-18 Antibody (6F7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDH18 (NP_004925, 467 a.a. ~ 576 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DLLSHVTVGIRVLDVNDNPPELAREYDIIVCENSKPGQVIHTISATDKDDFANGPRFNFFLDERLPVNPNFTLKDNEDNTASILTRRRRFSRTVQDVYYLPIMISDGGIP
• Specificity: CDH18 - cadherin 18, type 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH18
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cadherin-18 Antibody (6F7) - Azide and BSA Free

• cadherin 18, type 2
• cadherin-14
• cadherin-18
• cadherin-like 24
• CDH14EY-CADHERIN
• CDH14L
• CDH24
• ey-cadherin

Background

This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-18 Antibody (H00001016-M01)(1)

Publication using H00001016-M01

• Klein ME, Dickson MA, Antonescu C et al. PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Oncogene 2018-05-23 [PMID: 29789718]

Bioinformatics

• Gene Symbol: CDH18
• Entrez:
  • 1016(Human)
• OMIM:
  • 603019(Human)
• Uniprot:
  • NP_004925(Human)