Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 0.1-0.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Cadherin-10 protein
Human Cadherin-10
(Glu23-Ala613)
Accession # Q9Y6N8
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu23 & Gly55
Protein/Peptide Type
Recombinant Proteins
Gene
CDH10
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
92.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-120 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cadherin-10 (CAD-10) Fc Chimera, CF

  • CAD10
  • cadherin 10, type 2 (T2-cadherin)
  • Cadherin10
  • Cadherin-10
  • CDH10
  • T2-Cadherin

Background

Cadherin-10, also known as T2-Cadherin, is an approximately 120 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human Cadherin-10 is synthesized with a 22 amino acid (aa) signal peptide and a 32 aa N‑terminal propeptide. The mature cell surface-expressed protein consists of a 559 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3, 4). Within the propeptide and ECD, human Cadherin-10 shares 97% aa sequence identity with mouse and rat Cadherin-10. It interacts homotypically as well as hetertypically with Cadherin-9 and more weakly with Cadherin-6 (4). Cadherin-10 is expressed in the central nervous system, particularly in the limbic system, frontal cortex, somatosensory barrel cortex (in rat), olfactory structures, epithelial cells lining the choroid plexus and ventricles, retinal pigmented epithelium, and endothelial cells of the brain and retinal microvasculature (3, 5‑10). It is also expressed on epithelial luminal cells of the prostate, as well as in testes, ovary, placenta, kidney, small intestine, and on CD4+CD8+ T cells (3, 10‑13). Cadherin-10 expression is decreased postnatally in the barrel cortex and testes (8, 12). It is also down‑regulated in glioblastoma vasculature and prostate cancer (10, 11). Polymorphisms of human Cadherin-10 are associated with autism spectrum disorders (6).
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Kools, P. et al. (1999) FEBS Lett. 452:328.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Fushimi, D. et al. (1997) Dev. Dyn. 209:269.
  6. Wang, K. et al. (2009) Nature 459:528.
  7. Bekirov, I.H. et al. (2002) Neuroscience 115:213.
  8. Gil, O.D. et al. (2002) J. Comp. Neurol. 453:372.
  9. Akins, M.R. et al. (2007) J. Comp. Neurol. 501:483.
  10. Williams, M.J. et al. (2005) Brain Res. 1058:62.
  11. Walker, M.M. et al. (2008) Mod. Pathol. 21:85.
  12. Munro, S.B. and O.W. Blaschuk (1996) Biol. Reprod. 55:822.
  13. Munro, S.B. et al. (1996) Cell. Immunol. 169:309.

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Bioinformatics

Gene Symbol CDH10
Entrez
Uniprot