Cadherin-13 Antibody [Unconjugated]

Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cadherin-13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Cadherin-13 was detected in immersion fixed NCI-H460 human large cell lung carcinoma cell line using 10 µg/mL Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cadherin-13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 7A3 cells.(A) Cell lysates from 46BR.1G1 and 7A3 cells were analyzed by Western blotting with anti-cadherin 13, anti-cadherin 4, and anti-alpha -tubulin antibodies. (B) Quantification of the assay was performed by densitometric analysis with NIH ImageJ 1.43 program. Bars show mean ± SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 31W cells.Cell lysates from 46BR.1G1 and 31W cells were analyzed by Western blotting with antibodies against the indicated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license. Not internally tested by R&D Systems.

HIF-1 activation-induced T-cadherin upregulation increased adiponectin accumulation and EV production. (A) Western blot analysis of total cell lysates. UV-F2 cells cultured in DMEM containing 5% serum from adiponectin knockout mice were treated with or without high molecular weight adiponectin (HMW-APN) (10 μg/mL) and roxadustat (Roxa) (50 μM) for 48 h (n = 3 for each group). (B) Western blot analysis of EVs isolated from cell culture medium by differential ultracentrifugation. UV-F2 cells cultured in FBS-free Advanced DMEM were treated with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). Alix, TSG101, and syntenin were evaluated as EV markers. (C) Western blot analysis of total cell lysates. UV-F2 cells transfected control (Cont) or T-cadherin (T-cad) siRNA were cultured in DMEM containing 5% serum from adiponectin knockout mice with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). (D) Western blot analysis of EVs isolated from cell culture medium. UV-F2 cells transfected Cont or T-cad siRNA were cultured in FBS-free Advanced DMEM with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3). Data are means ± SEMs. *p AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cadherin-13 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cadherin-13
  Glu23-Ala692
  Accession # P55290
• Specificity: Detects human Cadherin-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-13 Antibody [Unconjugated]

• cadherin 13, H-cadherin (heart)
• Cadherin13
• Cadherin-13
• CDH13
• CDHHT-cadherin
• H-Cadherin
• Heart cadherin
• heart-cadherin
• P105
• T-cad
• T-Cadherin
• Truncated cadherin
• truncated-cadherin

Background

Cadherin-13, also known as T-Cadherin and H-Cadherin, is a 105 kDa member of the cadherin family of transmembrane glycoproteins that mediate calcium-dependent intercellular adhesion (1). However, Cadherin-13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin-13 to the plasma membrane (1-2). Human Cadherin-13 is synthesized as a 713 amino acid (aa) precursor that contains a 22 aa signal sequence, a 116 aa propeptide, a 555 aa mature chain, and a second propeptide of 20 aa that is removed in the mature form to reveal the GPI anchor. The mature form contains five cadherin domains and eight potential sites for N-linked glycosylation. Mature human Cadherin-13 shares 96% aa identity with mature mouse Cadherin-13. Cadherin-13 is expressed in various tissues. It is highly expressed in the heart, and in the CNS, Cadherin-13 is expressed in the cerebral cortex, medulla, hippocampus, amygdala, thalamus, and substantia nigra (2). There are higher levels of Cadherin-13 in the adult brain than in developing brain (2). Cadherin-13 is also expressed in skin in the basal layer of the epidermis, lung, liver, kidney, and blood vessels (2). The structural characteristics of Cadherin-13 predict that it is unlikely to function as a true adhesion molecule in vivo (2). It is suggested that it may act rather as a signaling receptor participating in recognition of the environment and regulation of cell motility, proliferation, and phenotype (2). Cellular expression levels of Cadherin-13 in various tissues often correlate, negatively or positively, with the proliferative potential of the cells (2). Cadherin-13 may also act as a suppressor of tumor cell growth (2). This potential role for Cadherin-13 was emphasized by localization of Cadherin-13 gene to chromosome 16q24, a region exhibiting loss of heterozygosity in many solid tumors (2). Allelic loss of chromosome bands 16q24.1-q24.2 and reduced expression of Cadherin-13, as well as hypermethylation of the remaining allele have been detected in a considerable number of human cancers (2).

1. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-13 Antibody (AF3264)(20)

Publications using AF3264

• Fujii, K;Fujishima, Y;Kita, S;Kawada, K;Fukuoka, K;Sakaue, TA;Okita, T;Kawada-Horitani, E;Nagao, H;Fukuda, S;Maeda, N;Nishizawa, H;Shimomura, I; Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin Scientific reports 2024-02-13 [PMID: 38351156] (Western Blot, Mouse)
• Keita Fukuoka, Ryohei Mineo, Shunbun Kita, Shiro Fukuda, Tomonori Okita, Emi Kawada-Horitani et al. ER stress decreases exosome production through adiponectin/T-cadherin-dependent and -independent pathways Journal of Biological Chemistry 9/1/2023 [PMID: 37524131]
• Tomonori Okita, Shunbun Kita, Shiro Fukuda, Keita Fukuoka, Emi Kawada-Horitani, Masahito Iioka et al. Soluble T-cadherin promotes pancreatic beta -cell proliferation by upregulating Notch signaling iScience 11/18/2022 [PMID: 36439986]
• MR Vitale, JEM Zöller, C Jansch, A Janz, F Edenhofer, E Klopocki, D van den Ho, T Vanmierlo, O Rivero, N Nadif Kasr, GC Ziegler, KP Lesch Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9 Stem Cell Research, 2021-01-11;51(0):102169. 2021-01-11 [PMID: 33486346] (ICC, Western Blot, Human)
• Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020-07-10;0(0):. 2020-07-10 [PMID: 32652045] (Western Blot, Human)
• Shunbun Kita, Shiro Fukuda, Norikazu Maeda, Iichiro Shimomura Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin eLife 10/24/2019 [PMID: 31647413]
• Stanislaw A. Buechner, Therese J. Resink T-Cadherin Expression in Actinic Keratosis Transforming to Invasive Squamous Cell Carcinoma Dermatopathology (Basel) 1/10/2019 [PMID: 31049318]
• Stanislaw A. Buechner, Therese J. Resink T-Cadherin Expression in Actinic Keratosis Transforming to Invasive Squamous Cell Carcinoma Dermatopathology (Basel) 2019-01-10 [PMID: 31049318] (Immunohistochemistry, Human)
• D Mori, S Miyagawa, R Matsuura, N Sougawa, S Fukushima, T Ueno, K Toda, T Kuratani, K Tomita, N Maeda, I Shimomura, Y Sawa Pioglitazone strengthen therapeutic effect of adipose-derived regenerative cells against ischemic cardiomyopathy through enhanced expression of adiponectin and modulation of macrophage phenotype Cardiovasc Diabetol, 2019-03-22;18(1):39. 2019-03-22 [PMID: 30902059] (Western Blot, Rat)
• Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release JCI Insight, 2018-04-19;3(8):. 2018-04-19 [PMID: 29669945] (ICC, Western Blot, Mouse)
• Andrea Forero, Olga Rivero, Sina Wäldchen, Hsing-Ping Ku, Dominik P. Kiser, Yvonne Gärtner et al. Cadherin-13 Deficiency Increases Dorsal Raphe 5-HT Neuron Density and Prefrontal Cortex Innervation in the Mouse Brain Frontiers in Cellular Neuroscience 9/26/2017 [PMID: 29018333]
• A Frismantie, E Kyriakakis, B Dasen, P Erne, TJ Resink, M Philippova Actin cytoskeleton regulates functional anchorage-migration switch during T-cadherin-induced phenotype modulation of vascular smooth muscle cells Cell Adh Migr, 2017-05-25;0(0):1-17. 2017-05-25 [PMID: 28524745] (Western Blot, Human)
• S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2 J. Biol. Chem, 2017-03-21;0(0):. 2017-03-21 [PMID: 28325833] (Western Blot, Mouse)
• Georgia M Alexander Vagal Nerve Stimulation Modifies Neuronal Activity and the Proteome of Excitatory Synapses of Amygdala/Piriform Cortex J. Neurochem, 2017-02-03;0(0):. 2017-02-03 [PMID: 27973753] (Western Blot, Rat)
• Stanislaw Buechner T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin Dermatopathology (Basel), 2016-10-21;3(4):68-78. 2016-10-21 [PMID: 27904857] (IHC, Human)
• Cremaschi P, Oliverio M, Leva V et al. Chronic Replication Problems Impact Cell Morphology and Adhesion of DNA Ligase I Defective Cells PLoS ONE. 7/8/2015 [PMID: 26151554]
  
  Details:
  Cadherin-4/R-Cadherin antibody was used at 1:300 dilution for WB assay on lysates from Human SV40-transformed 46BR.1G1 fibroblasts, 7A3 cells and 31W cells (Figure 5 and 6).
• Thegna Mavroconstanti, Stefan Johansson, Ingeborg Winge, Per M. Knappskog, Jan Haavik Functional Properties of Rare Missense Variants of Human CDH13 Found in Adult Attention Deficit/Hyperactivity Disorder (ADHD) Patients PLoS ONE 8/1/2013 [PMID: 23936508]
• Parker-Duffen , Jennifer, Nakamura , Kazuto, Silver , Marcy, Kikuchi , Ryosuke, Tigges , Ulrich, Yoshida , Sumiko, Denzel , Martin S, Ranscht , Barbara, Walsh , Kenneth T-cadherin is essential for adiponectin-mediated revascularization. J Biol Chem, 2013-07-03;288(34):24886-97. 2013-07-03 [PMID: 23824191] (IHC-Fr, Immunoprecipitation, Western Blot, Mouse)
• Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, Erne P, Resink T, Philippova M Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts. Cell Signal, 2013-02-11;25(5):1044-53. 2013-02-11 [PMID: 23411345] (ICC, Western Blot, Human)
• Philippova M, Ivanov D, Joshi MB, Kyriakakis E, Rupp K, Afonyushkin T, Bochkov V, Erne P, Resink TJ Identification of proteins associating with glycosylphosphatidylinositol- anchored T-cadherin on the surface of vascular endothelial cells: role for Grp78/BiP in T-cadherin-dependent cell survival. Mol. Cell. Biol., 2008-04-14;28(12):4004-17. 2008-04-14 [PMID: 18411300] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: CDH13
• Uniprot:
  • P55290()