CAB39 Antibody - Azide and BSA Free

Western Blot: CAB39 Antibody [H00051719-B01P] - Analysis of CAB39 expression in transfected 293T cell line by CAB39 polyclonal antibody. Lane 1: CAB39 transfected lysate(37.51 KDa). Lane 2: Non-transfected lysate.

Proximity Ligation Assay: CAB39 Antibody [H00051719-B01P] - Analysis of protein-protein interactions between STK11 and CAB39. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-CAB39 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein in

• Reactivity: Hu
• Applications: WB, Func
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CAB39 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CAB39 Antibody - Azide and BSA Free (H00051719-B01P) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CAB39 (NP_057373.1, 1 a.a. - 341 a.a.) full-length human protein. MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA
• Specificity: CAB39 - calcium binding protein 39,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CAB39
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CAB39 Antibody - Azide and BSA Free

• calcium binding protein 39
• calcium-binding protein 39
• FLJ22682
• MO25CGI-66
• Protein Mo25

Background

Mouse protein 25 alpha (MO25 alpha) is a 40-kDa protein that, together with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase, forms a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase. The latter is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25 alpha binds directly to a conserved Trp-Glu-Phe sequence at the STRAD alpha C terminus, markedly enhancing binding of STRAD alpha to LKB1 and increasing LKB1 catalytic activity (1). Skeletal muscle contraction results in the phosphorylation and activation of the AMP-activated protein kinase (AMPK) by an upstream kinase (AMPKK). The LKB1-STE-related adaptor (STRAD)-mouse protein 25 (MO25) complex is the major AMPKK in skeletal muscle; however, LKB1-STRAD-MO25 activity is not increased by muscle contraction. This relationship suggests that phosphorylation of AMPK by LKB1-STRAD-MO25 during skeletal muscle contraction may be regulated by allosteric mechanisms (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAB39
• Entrez:
  • 51719(Human)
• Uniprot:
  • NP_057373.1(Human)