CA125/MUC16 Antibody (X75)

Images

 
Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (X75) [NB600-1468] - Analysis in in urinary bladder adenocarcinoma.
Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (X75) [NB600-1468] - Analysis in lung adenocarcinoma.
Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (X75) [NB600-1468] - Analysis in antigen in serosal ovarian adenocarcinoma.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, S-ELISA
Clone
X75
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CA125/MUC16 Antibody (X75) Summary

Immunogen
CA-125 purified from human ovarian carcinoma.
Localization
Cell Membrane
Specificity
This specifically reacts with human ovarian carcinoma cells; specifically epitope specificity group B (ISOBM classification).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC16
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA 1:100-1:2000
Application Notes
Western blot: use at a concentration of 1-5ug/ml after electrophoresing 20 ug of sample. Optimal dilutions / concentrations should be determined by the end user. Dilute in PBS or medium which is identical to that used in the assay system. Suggested pairs for a sandwich immunoassay of CA125 antigen (capture-detection): NB120-10032 (capture) with NB600-1468 as detection.
Publications
Read Publications using NB600-1468.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM Tris (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Purity
Ion exchange chromatography

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for CA125/MUC16 Antibody (X75)

  • CA125 ovarian cancer antigen
  • CA125
  • CA-125
  • CA125MUC-16
  • FLJ14303
  • MUC16
  • mucin 16, cell surface associated
  • mucin-16
  • Ovarian cancer-related tumor marker CA125
  • Ovarian carcinoma antigen CA125

Background

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller 'subunits' being described by some investigators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CA125/MUC16 Antibody (NB600-1468)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CA125/MUC16 Antibody (NB600-1468). (Showing 1 - 1 of 1 FAQs).

  1. We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
    • These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MUC16
Entrez
Uniprot