CA125/MUC16 Antibody (X75)


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Product Details

Reactivity HuSpecies Glossary
Applications WB, S-ELISA

Order Details

CA125/MUC16 Antibody (X75) Summary

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
Cell Membrane
CA125 antigen. This specifically reacts with human ovarian carcinoma cells. Epitope specificity group B (ISOBM classification) similar to M-11.
Ion exchange chromatography
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  • Western Blot 1-5 ug/ml
  • Sandwich ELISA 1:100-1:2000
Application Notes
Western blot: use at a concentration of 1-5ug/ml after electrophoresing 20 ug of sample. Optimal dilutions / concentrations should be determined by the end user. Dilute in PBS or medium which is identical to that used in the assay system. Suggested pairs for a sandwich immunoassay of CA125 antigen (capture-detection): NB120-10032 (capture) with NB600-1468 as detection.
Read Publications using NB600-1468.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
10mM Tris (pH 7.5) and 0.15M NaCl
0.05% Sodium Azide
Ion exchange chromatography


Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at for more details.

Alternate Names for CA125/MUC16 Antibody (X75)

  • CA125 ovarian cancer antigen
  • CA125
  • CA-125
  • CA125MUC-16
  • FLJ14303
  • MUC16
  • mucin 16, cell surface associated
  • mucin-16
  • Ovarian cancer-related tumor marker CA125
  • Ovarian carcinoma antigen CA125


MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller 'subunits' being described by some investigators.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CA125/MUC16 Antibody (NB600-1468)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CA125/MUC16 Antibody (NB600-1468). (Showing 1 - 1 of 1 FAQs).

  1. We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
    • These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.

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Gene Symbol MUC16