CA125/MUC16 Antibody (OV185-1)

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (OV185-1) [NBP1-22564] - Formalin fixed paraffin embedded human ovarian cancer stained with CA125 antibody (NBP1-22564).

• Reactivity: Hu
• Applications: IHC
• Clone: OV185-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CA125/MUC16 Antibody (OV185-1) Summary

• Immunogen: Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian cancer.
• Localization: Extracellular membrane
• Specificity: This antibody reacts specifically with CA125 ovarian cancer antigen. Several studies have shown that CA125 is a useful tumor marker for ovarian epithelial malignancies. However, CA125 has been described in other neoplasms such as seminal vesicle and anaplastic lymphomas.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC16
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CA125/MUC16 Antibody (OV185-1)

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller subunits being described by some investigators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CA125/MUC16 Antibody (NBP1-22564)(2)

Publications using NBP1-22564

• Israelsson P, BjOrk E, Nagaev I et al. NKG2D-mediated cytotoxicity improves after primary surgery for high-grade serous ovarian cancer American journal of reproductive immunology (New York, N.Y. : 1989) 2022-11-06 [PMID: 36335434] (EM, Human)
• Labani-Motlagh A, Israelsson P, Ottander U et al. Differential expression of ligands for NKG2D and DNAM-1 receptors by epithelial ovarian cancer-derived exosomes and its influence on NK cell cytotoxicity. Tumour Biol. 2015-11-13 [PMID: 26563374]

Bioinformatics

• Gene Symbol: MUC16
• Uniprot:
  • Q8WXI7()