C1qTNF5/CTRP5 Antibody [Unconjugated]

A172 human glioblastoma cell line was stained with Goat Anti-Human CTRP5/C1qTNF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3167, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

C1qTNF5/CTRP5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CTRP5/C1qTNF5
  Ser16-Ala243
  Accession # AAH29485
• Specificity: Detects human CTRP5/C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CTRP4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: C1QTNF5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C1qTNF5/CTRP5 Antibody [Unconjugated]

• C1q and tumor necrosis factor related protein 5
• C1qTNF5
• Complement C1q Tumor Necrosis Factor-Related Protein 5 Precursor Variant 3
• CTRP5
• CTRP5LORDDKFZp586B0621
• LORD 6
• Myonectin 3

Background

C1qTNF5, also known as CTPR5, is a secreted protein that belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF-related protein family. All family members share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a
C‑terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse C1qTNF5 share 94% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C1qTNF5/CTRP5 Antibody (AF3167)(1)

Publication using AF3167

• Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions. Biochem. J., 2008-12-01;416(2):161-77. 2008-12-01 [PMID: 18783346] (Western Blot, Human)

Bioinformatics

• Gene Symbol: C1QTNF5
• Uniprot:
  • AAH29485()