c-Abl Antibody

Western Blot: c Abl Antibody [NB110-81723] - Detection of C-ABL in K562 whole cell lysate using NB110-81723.

Immunocytochemistry/Immunofluorescence: c Abl Antibody [NB110-81723] - ICC/IF staining of c-Abl in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

c-Abl Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human c Abl (within residues 700-800). [Swiss-Prot# P00519]
• Localization: Cytoplasm, cytoskeleton. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Western Blot 2 ug/ml
• Application Notes: This c Abl antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~120 kDa. In ICC/IF cytoplasmic staining is observed in untreated cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  K-562 Whole Cell Lysate
  c-Abl Overexpression Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for c-Abl Antibody

• Abelson murine leukemia viral oncogene homolog 1
• ABL
• ABL1
• bcr/abl
• BCR-ABL1
• c-abl oncogene 1, non-receptor tyrosine kinase
• c-abl oncogene 1, receptor tyrosine kinase
• cAbl
• c-Abl
• EC 2.7.10
• EC 2.7.10.2
• JTK7
• JTK7bcr/abl
• p150bcr/c-abl oncogene protein
• Proto-oncogene c-Abl
• proto-oncogene tyrosine-protein kinase ABL1
• tyrosine-protein kinase ABL1
• v-abl Abelson murine leukemia viral oncogene homolog 1
• v-abl

Background

c-ABL regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. It localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Activity of the c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. c-ABL also potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABL1
• Entrez:
  • 25(Human)
  • 11350(Mouse)
• Uniprot:
  • P00519(Human)
  • P00520(Mouse)