Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Butyrylcholinesterase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Butyrylcholinesterase antibody, and then analyzed by flow cytometry.

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Butyrylcholinesterase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Butyrylcholinesterase.

• Reactivity: Hu, Ca
• Applications: WB, Flow
• Clone: OTI1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.72 mg/ml

Butyrylcholinesterase/BCHE Antibody (OTI1E1) Summary

• Immunogen: Full length human recombinant protein of human BCHE (NP_000046) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCHE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 68.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.72 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

• Acylcholine acylhydrolase
• BCHE
• Butyrylcholine esterase
• Butyrylcholinesterase
• CHE1
• CHE1cholinesterase
• Choline esterase II
• cholinesterase 1
• E1
• EC 3.1.1.8
• Pseudocholinesterase

Background

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCHE
• Entrez:
  • 590(Human)