![Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BUD13 antibody NBP1-81863 (A) shows similar protein distribution across tissues to independent antibody NBP2-38407 (B).](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunohistochemistry-Paraffin-NBP1-81863-img0010.jpg "Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BUD13 antibody NBP1-81863 (A) shows similar protein distribution across tissues to independent antibody NBP2-38407 (B).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit BUD13 Antibody - BSA Free (NBP1-81863) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PGHQDSDSDLSPPRNRPRHRSSDSDLSPPRRRQRTKSSDSDLSPPRRSQPPGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETM |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BUD13 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BUD13 |
![Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/BUD13-Antibody-Western-Blot-NBP1-81863-img0007.jpg "Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: BUD13 Antibody [NBP1-81863] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81863-img0008.jpg "Immunocytochemistry/Immunofluorescence: BUD13 Antibody [NBP1-81863] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human colon.](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunohistochemistry-Paraffin-NBP1-81863-img0013.jpg "Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human colon.")
![Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/BUD13-Antibody-Western-Blot-NBP1-81863-img0006.jpg "Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunohistochemistry-Paraffin-NBP1-81863-img0011.jpg "Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human testis.](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunohistochemistry-Paraffin-NBP1-81863-img0012.jpg "Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human testis.")
![Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human kidney.](http://images.novusbio.com/fullsize/BUD13-Antibody-Immunohistochemistry-Paraffin-NBP1-81863-img0014.jpg "Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human kidney.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")