Bub3 Antibody - BSA Free

Western Blot: Bub3 Antibody [NBP1-76796] - Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-76796] - Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-76796] - Jurkat cells with Bub3 antibody at 10 ug/ml.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bub3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bub3 Antibody - BSA Free (NBP1-76796) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3. The immunogen is located within the last 50 amino acids of Bub3. Amino Acid Squence: SYMYEMDDTEHPEDG
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BUB3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  Bub3 Peptide (NBP1-76796PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bub3 Antibody - BSA Free

• BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
• BUB3 budding uninhibited by benzimidazoles 3 homolog
• Bub3
• BUB3L
• budding uninhibited by benomyl
• budding uninhibited by benzimidazoles 3 homolog (yeast)
• hBUB3
• mitotic checkpoint component
• mitotic checkpoint protein BUB3

Background

The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB3
• Uniprot:
  • O43684()