Bub1 Knockout HeLa Cell Lysate

Images

 
There are currently no images for Bub1 Lysate (NBP2-77080).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

Bub1 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4
Gene
BUB1

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Bub1 Knockout HeLa Cell Lysate

  • BUB1 budding uninhibited by benzimidazoles 1 homolog
  • Bub1
  • BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
  • BUB1L
  • budding uninhibited by benzimidazoles 1 (yeast homolog)
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
  • EC 2.7.11.1
  • hBUB1mitotic spindle checkpoint kinase
  • putative serine/threonine-protein kinase

Background

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-563
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-353
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NBP1-88626
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-75424
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-89979
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32775
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF6028
Species: Hu
Applications: WB, IHC
NB100-59828
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1116
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-56479
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1103
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
NBP1-81765
Species: Hu
Applications: IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-294
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, MiAr
NBP1-82875
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-24509
Species: Hu, Mu, Rt, Bv, Ca, Op, Pm
Applications: WB, IHC, IHC-P
NBP2-77080
Species: Hu
Applications: WB

Publications for Bub1 Lysate (NBP2-77080) (0)

There are no publications for Bub1 Lysate (NBP2-77080).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Bub1 Lysate (NBP2-77080) (0)

There are no reviews for Bub1 Lysate (NBP2-77080). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bub1 Lysate (NBP2-77080) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-77080

Additional Bub1 Products

Bioinformatics Tool for Bub1 Lysate (NBP2-77080)

Discover related pathways, diseases and genes to Bub1 Lysate (NBP2-77080). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Bub1 Lysate (NBP2-77080)

Discover more about diseases related to Bub1 Lysate (NBP2-77080).
 

Pathways for Bub1 Lysate (NBP2-77080)

View related products by pathway.

PTMs for Bub1 Lysate (NBP2-77080)

Learn more about PTMs related to Bub1 Lysate (NBP2-77080).
 

Research Areas for Bub1 Lysate (NBP2-77080)

Find related products by research area.

Blogs on Bub1

There are no specific blogs for Bub1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Bub1 Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol BUB1