Bub1 Antibody (14H5) [DyLight 488]

Product Discontinued

Bub1 Antibody (14H5) [DyLight 488] Summary

• Immunogen: Derived from the hybridoma 14H5 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with recombinant human BUB1.
• Specificity: Recognizes human BUB1 at a band of approximately 150kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BUB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Reactivity Notes

Cross-reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bub1 Antibody (14H5) [DyLight 488]

• BUB1 budding uninhibited by benzimidazoles 1 homolog
• Bub1
• BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
• BUB1L
• budding uninhibited by benzimidazoles 1 (yeast homolog)
• budding uninhibited by benzimidazoles 1 homolog (yeast)
• EC 2.7.11.1
• hBUB1mitotic spindle checkpoint kinase
• putative serine/threonine-protein kinase

Background

BUB1 encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1