BTN2A2/Butyrophilin 2 Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue, human heart tissue, and human testis tissue. PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BTN2A2/Butyrophilin 2A2 was detected in immersion fixed paraffin-embedded sections of human thymus using Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) at a 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 60 kDa (as indicated) using 1:25 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BTN2A2/Butyrophilin 2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human BTN2A2/Butyrophilin 2A2
  Phe34-Val237
  Accession # Q8WVV5
• Specificity: Detects human BTN2A2/Butyrophilin 2A2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse BTN2A2 is observed, less than 5% cross-reactivity with recombinant human (rh) BTN2A1 and rhBTN3A2 is observed, and less than 1% cross-reactivity with rhBTN1A1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTN2A2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 dilution
  • Simple Western 1:25 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTN2A2/Butyrophilin 2 Antibody [Unconjugated]

• BTF2
• BTF2BT2.2butyrophilin 2
• BTN2
• BTN2A2
• Butyrophilin 2
• butyrophilin subfamily 2 member A2
• butyrophilin, subfamily 2, member A2
• FLJ41908

Background

Butyrophilin subfamily 2 member A2 (BTN2A2) is a protein encoded by the BTN2A2 gene and is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type 1 receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants of this gene have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTN2A2
• Uniprot:
  • Q8WVV5(Human)