BTF3 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SK-BR-3 human breast cancer cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BTF3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BTF3 was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BTF3 Antibody Summary

• Immunogen: E. coli-derived recombinant human BTF3
  Lys46-Asn206
  Accession # P20290
• Specificity: Detects human, mouse and rat BTF3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BTF3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTF3 Antibody

• basic transcription factor 3
• BETA-NAC
• BTF3
• BTF3a
• BTF3b
• NACB
• nascent-polypeptide-associated complex beta polypeptide
• RNA polymerase B transcription factor 3
• transcription factor BTF3

Background

BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex beta /NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ER alpha ) stimulation of gene activation by binding to both ligand activated and non-ligand activated ER alpha . Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTF3 Antibody (AF5747)(2)

Publications using AF5747

• Long Liu, Yanfeng Fan, Debiao Zhao, Jason Ioannidis, Daoqing Gong, Michael Clinton Expression Profile of Chicken Sex Chromosome Gene<b><i> BTF3 </i></b>is Linked to Gonadal Phenotype Sexual Development 1/31/2020 [PMID: 32155647]
• Aydan Bulut-Karslioglu, Trisha A. Macrae, Juan A. Oses-Prieto, Sergio Covarrubias, Michelle Percharde, Gregory Ku et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output Cell Stem Cell 2018-03-01 [PMID: 29499153] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BTF3
• Uniprot:
  • P20290()