BTK Antibody - BSA Free

Western Blot: BTK Antibody [NBP1-76596] - Analysis of BTK in U937 cell lysate with anti-BTK using NBP1-76596 at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-76596] - Daudi cells with BTK antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596] - Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 ug/mL.

Immunohistochemistry: BTK Antibody - BSA Free [NBP1-76596] - Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596] - Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 ug/ml.Red: BTK Antibody Blue: DAPI staining

• Reactivity: Hu, Mu, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BTK Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK. The immunogen is located within amino acids 20 - 70 of BTK. Amino Acid Squence: SYYEYDFERGRRGSKK
• Specificity: Multiple isoforms of BTK are known to exist.
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTK
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  BTK Peptide (NBP1-76596PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BTK Antibody - BSA Free

• Agammaglobulinaemia tyrosine kinase
• AGMX1
• AGMX1MGC126262
• AT
• ATKIMD1
• B-cell progenitor kinase
• BPK
• Bruton agammaglobulinemia tyrosine kinase
• Bruton tyrosine kinase
• BTK
• dominant-negative kinase-deficient Brutons tyrosine kinase
• EC 2.7.10
• EC 2.7.10.2
• IMD1
• PSCTK1
• tyrosine-protein kinase BTK
• XLA
• XLAMGC126261

Background

Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTK
• Uniprot:
  • Q06187()