BTK Antibody (7F12H4)


Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract.
Immunocytochemistry/ Immunofluorescence: BTK Antibody (7F12H4) [NB110-87047] - The BTK antibody was tested in A431 cells against DyLight 488 (Green). Actin and nuclei were counterstained for Phalloidin (Red) and DAPI more
Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) [NB110-87047] - Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and more
Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 1) truncated BTK recombinant protein and 2) K562 cell lysate.

Product Details

Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1.0 mg/ml

Order Details

BTK Antibody (7F12H4) Summary

Purified recombinant C terminal fragment of BTK expressed in E. coli. [UniProt# Q06187]
Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note: In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
Ammonium sulfate precipitation
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
1.0 mg/ml
Ammonium sulfate precipitation


Application Notes
This BTK (7F12H4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
77 kDa

Reactivity Notes

Human and monkey.

Alternate Names for BTK Antibody (7F12H4)

  • Agammaglobulinaemia tyrosine kinase
  • AGMX1
  • AGMX1MGC126262
  • AT
  • B-cell progenitor kinase
  • BPK
  • Bruton agammaglobulinemia tyrosine kinase
  • Bruton tyrosine kinase
  • dominant-negative kinase-deficient Brutons tyrosine kinase
  • EC 2.7.10
  • EC
  • IMD1
  • PSCTK1
  • tyrosine-protein kinase BTK
  • XLA
  • XLAMGC126261


BTK, also known as Brutons tyrosine kinase, is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BTK Antibody (NB110-87047). (Showing 1 - 1 of 1 FAQ).

  1. Could you please let me know the concentration of this antibody?
    • This product is currently supplied as unpurified ascites, so the antibody concentration is unknown.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.

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Gene Symbol BTK

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