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BTBD12 Antibody

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Western Blot: BTBD12 Antibody [H00084464-B01P] - Analysis of BTBD12 expression in transfected 293T cell line by BTBD12 polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.
Western Blot: BTBD12 Antibody [H00084464-B01P] - Analysis of BTBD12 expression in transfected 293T cell line by BTBD12 polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, KD
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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BTBD12 Antibody Summary

Immunogen
BTBD12 (AAH36335.1, 1 a.a. - 1151 a.a.) full-length human protein. MVNNPHLSDVQFQTDSGEVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN
Specificity
BTBD12
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SLX4
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Use in Knockdown Validated reported in scientific literature (PMID: 31067464).
Reviewed Applications
Read 1 Review rated 4
using
H00084464-B01P in the following applications:
Publications
Read Publications using
H00084464-B01P in the following applications:

  • KD
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31067464).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BTBD12 Antibody

  • BTB (POZ) domain containing 12
  • BTB/POZ domain-containing protein 12
  • BTBD12
  • BTBD12structure-specific endonuclease subunit SLX4
  • FANCP
  • KIAA1784
  • KIAA1784MUS312
  • KIAA1987
  • KIAA1987FANCP
  • SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
  • SLX4

Background

BTBD12 (BTBD12 domain-containing protein 12) has been identified as the human ortholog of the yeast Slx4p and drosophila MUS312 DNA repair factors. BTBD12 is an ATM/ATR checkpoint substrate that functions as part of a Holliday junction resolvase and is important for DNA interstrand crosslink repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.
Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Western Blot: Mus81 Antibody (mta30 2g10/3) [NB100-2064] - Detection of Mus81 in HeLa whole cell lysate using NB100-2064.
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Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IP, WB
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Immunocytochemistry/Immunofluorescence: EME1 Antibody [H00146956-B01P] - Analysis of purified antibody to EME1 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: EME1 Antibody [H00146956-B01P] - Analysis of EME1 expression in HeLa.
Western Blot: EME1 Antibody [H00146956-B01P] - Analysis of EME1 expression in transfected 293T cell line by EME1 polyclonal antibody. Lane 1: EME1 transfected lysate(64.13 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: ICC/IF, WB
ATR Antibody (2B5)
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Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.
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Applications: Func, ICC/IF, IP, In vitro, KO, WB
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Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Anti-ERCC1 immunohistochemistry on whole sections of a lung squamous cell carcinoma, using Mab 8F1 and D-10 with CC1-mono and H2-60 protocols. Note increased cytosolic background with H2-60. Arrow: Necrotic centre. Arrowhead: Stromal axis. 100 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Cyto-histologic comparison of anti-ERCC1 immunoreactivity using the protocol 8F1 CC1-mono (top) or 8F1 H2-60 (bottom). Left: Pleural effusion sediment of lung adenocarcinoma. Right: thoracic lymph node. Arrow: Lymphocyte. Arrowhead: Streak of endothelial cells. Asterisk: Tumor cell cluster. 400 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
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Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of ATM Antibody [NB100-104PE]. An intracellular stain was performed on HeLa cells with ATM antibody [NB100-104PE] (blue) and a matched isotype control [NBP2-24893PE] (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone with ATM Antibody [NB100-104].
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HepG2 cells with PE-conjugated ATM antibody [NB100-104PE] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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Western Blot: GEN1 Antibody [NBP2-30783] - Analysis in human cell line RH-30.
Immunocytochemistry/Immunofluorescence: GEN1 Antibody [NBP2-30783] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Species: Hu
Applications: ICC/IF, WB
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Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.
Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [NB100-598] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow Cytometry: BRCA1 Antibody (RAY) [NB100-598] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.
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Immunocytochemistry/Immunofluorescence: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human cell line A-431 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.
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BSA Free
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Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NB110-57130] - RNAi-mediated depletion of human separase (ESPL1) induces TIFs. Control scrambled siRNA- (control) and ESPL1 siRNA-treated fibroblasts stained with anti-p53-binding protein 1 (53BP1; green) and anti-TRF2 (red). It is noteworthy that in ESPL1 siRNA-treated cells, 53BP1 signals frequently overlap with TRF2 signals marking the TIFs. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms10405), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NB110-57130] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TRF-2 Antibody NB110-57130 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Flow Cytometry: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Western Blot: RRAD Antibody [NBP2-27500] - Panel A: Staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) with antibody at 0.5 ug/mL, and compared with an HA-specific antibody. Panel B: Staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.
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Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01J), clone 1G2. Lane 1: RAD1 transfected lysate(32.43 KDa).Lane 2: Non-transfected lysate.
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Detection against Immunogen (35.53 KDa) .
Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J] - Immunoperoxidase of monoclonal antibody to Rad1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
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Applications: ELISA, IHC, IHC-P, KD, ELISA(Cap), S-ELISA, WB
BRIP1/FANCJ
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Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
(4 Publications)
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Applications: ICC/IF, IHC, IHC-P, PLA, WB
Fascin Antibody
(833223) [Unconjugate
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Fascin at approximately 61 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.
Fascin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications: IHC, KO, Simple Western, WB
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Immunoprecipitation: MTMR15 Antibody [NBP1-42677] - Detection of human MTMR15 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTMR15 antibody NBP1-42677 used for IP at 6 ug per reaction. For blotting immunoprecipitated MTMR15, NBP1-42677 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: MTMR15 Antibody [NBP1-42677] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MTMR15 (NBP1-42677) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: MTMR15 Antibody [NBP1-42677] - Detection of human MTMR15 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HeLa, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTMR15 antibody NBP1-42677 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(1 Review)
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Applications: IHC, IHC-P, IP, WB
TEM8/ANTXR1
Antibody (200C1339(SB ...
NB100-56585
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
(5 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MMAB Antibody
(OTI2G5)
NBP2-03417
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - Analysis of Jurkat cells, using anti-MMAB antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
TBP like protein
TLP Antibody
NBP2-49671
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP2-49671] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP2-49671] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP2-49671] - Staining in human testis and liver tissues using anti-TBPL1 antibody. Corresponding TBPL1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
BTBD12 Antibody
H00084464-B01P
BTBD12 Antibody
Species: Hu, Mu
Applications: WB, ICC/IF, KD

Publications for BTBD12 Antibody (H00084464-B01P)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: KD.


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Review for BTBD12 Antibody (H00084464-B01P) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00084464-B01P:
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Images Ratings Applications Species Date Details
  4
reviewed by:
Bingbing Wan
 WB Human 12/14/2010
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells, Sample Amount: 30ug
SpeciesHuman
Lot09145WU1z

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Recent Reviews

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 Bingbing Wan
12/14/2010
Application: WB
Species: Human
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Bioinformatics

Gene Symbol SLX4
Entrez