BRM Knockout HeLa Cell Lysate

Product Discontinued

BRM Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-4 bp deletion in exon2 and -1 bp deletion in exon2 and 1 bp insertion in exon2
• Gene: SMARCA2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for BRM Knockout HeLa Cell Lysate

• ATP-dependent helicase SMARCA2
• BAF190B
• BRG1-associated factor 190B
• BRM
• BRMFLJ36757
• EC 3.6.1
• EC 3.6.4.-
• global transcription activator homologous sequence
• hBRM
• hBRMMGC74511
• hSNF2a
• probable global transcription activator SNF2L2
• Protein brahma homolog
• SMARCA2
• SNF2
• SNF2/SWI2-like protein 2
• SNF2A
• SNF2-alpha
• SNF2L2
• SNF2L2BAF190
• SNF2LA
• SNF2-like 2
• Sth1p
• subfamily a, member 2
• sucrose nonfermenting 2-like protein 2
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 2
• SWI2

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA2