Brg1 Antibody (CL13783) - Azide and BSA Free

Analysis in human testis and liver tissues using NBP3-43830 antibody. Corresponding Brg1 RNA-seq data are presented for the same tissues.

Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human small intestine shows strong nuclear positivity in glandular cells.

Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL13783
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Brg1 Antibody (CL13783) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Brg1 Antibody (CL13783) - Azide and BSA Free (NBP3-24576) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P51532, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Brg1 Antibody (CL13783) - Azide and BSA Free

• ATP-dependent helicase SMARCA4
• BAF190
• BAF190A
• brahma protein-like 1
• Brg1
• BRG1-associated factor 190A
• BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
• BRM/SWI2-related gene 1
• EC 3.6.1
• EC 3.6.4.-
• FLJ39786
• global transcription activator homologous sequence
• homeotic gene regulator
• hSNF2b
• Mitotic growth and transcription activator
• nuclear protein GRB1
• Protein brahma homolog 1
• Protein BRG-1
• RTPS2
• SMARCA4
• SNF2
• SNF2B
• SNF2-beta
• SNF2L4
• SNF2LB
• SNF2-like 4
• subfamily a, member 4
• sucrose nonfermenting-like 4
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI2
• transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA4