Brg1 Antibody (BRG1/7633R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with Brg1 antibody (BRG1/7633R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with Brg1 antibody (BRG1/7633R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: BRG1/7633R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Brg1 Antibody (BRG1/7633R) - Azide and BSA Free Summary

• Description: Positive Controls: HeLa or HEK-293T cells. Ubiquitous nuclear expression.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 200-400) of human Brg1 protein (exact sequence is proprietary)
• Localization: Nucleus.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMARCA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order Ab without BSA
  
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Brg1 Antibody (BRG1/7633R) - Azide and BSA Free

• ATP-dependent helicase SMARCA4
• BAF190
• BAF190A
• brahma protein-like 1
• Brg1
• BRG1-associated factor 190A
• BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
• BRM/SWI2-related gene 1
• EC 3.6.1
• EC 3.6.4.-
• FLJ39786
• global transcription activator homologous sequence
• homeotic gene regulator
• hSNF2b
• Mitotic growth and transcription activator
• nuclear protein GRB1
• Protein brahma homolog 1
• Protein BRG-1
• RTPS2
• SMARCA4
• SNF2
• SNF2B
• SNF2-beta
• SNF2L4
• SNF2LB
• SNF2-like 4
• subfamily a, member 4
• sucrose nonfermenting-like 4
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI2
• transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA4