Brg1 Antibody (BLR106H)

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696] - Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696] - Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit antiCOPB2 antibody.

Immunoprecipitation: Brg1 Antibody [NBP3-14696] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRG1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. BRG1 was alsoimmunoprecipitated by rabbit anti-BRG1 antibody. For blotting immunoprecipitated BRG1, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR106H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Brg1 Antibody (BLR106H) Summary

• Description: Novus Biologicals Rabbit Brg1 Antibody (BLR106H) (NBP3-14696) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: between 75 and 125
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMARCA4
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 20 ul/1mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for Brg1 Antibody (BLR106H)

• ATP-dependent helicase SMARCA4
• BAF190
• BAF190A
• brahma protein-like 1
• Brg1
• BRG1-associated factor 190A
• BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
• BRM/SWI2-related gene 1
• EC 3.6.1
• EC 3.6.4.-
• FLJ39786
• global transcription activator homologous sequence
• homeotic gene regulator
• hSNF2b
• Mitotic growth and transcription activator
• nuclear protein GRB1
• Protein brahma homolog 1
• Protein BRG-1
• RTPS2
• SMARCA4
• SNF2
• SNF2B
• SNF2-beta
• SNF2L4
• SNF2LB
• SNF2-like 4
• subfamily a, member 4
• sucrose nonfermenting-like 4
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI2
• transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA4