| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | RAY |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 350 |
| Immunogen | Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398] |
| Epitope | The epitope recognized is found between amino acids 1314-1600. |
| Localization | nuclear |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BRCA1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NB100-598AF350 | |
| Alexa Fluor 405 | NB100-598AF405 | |
| Alexa Fluor 488 | NB100-598AF488 | |
| Alexa Fluor 532 | NB100-598AF532 | |
| Alexa Fluor 594 | NB100-598AF594 | |
| Alexa Fluor 647 | NB100-598AF647 | |
| Alexa Fluor 700 | NB100-598AF700 | |
| Alexa Fluor 750 | NB100-598AF750 | |
| Allophycocyanin | NB100-598APC | |
| Biotin | NB100-598B | |
| DyLight 350 | NB100-598UV | |
| DyLight 405 | NB100-598V | |
| DyLight 488 | NB100-598G | |
| DyLight 550 | NB100-598R | |
| DyLight 594 | NB100-598DL594 | |
| DyLight 650 | NB100-598C | |
| DyLight 680 | NB100-598FR | |
| DyLight 755 | NB100-598IR | |
| FITC | NB100-598F | |
| HRP | NB100-598H | |
| Janelia Fluor 549 | NB100-598JF549 | |
| Janelia Fluor 646 | NB100-598JF646 | |
| PE | NB100-598PE |
Diseases for BRCA1 Antibody (NB100-598AF350)Discover more about diseases related to BRCA1 Antibody (NB100-598AF350).
| Pathways for BRCA1 Antibody (NB100-598AF350)View related products by pathway.
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PTMs for BRCA1 Antibody (NB100-598AF350)Learn more about PTMs related to BRCA1 Antibody (NB100-598AF350).
| Research Areas for BRCA1 Antibody (NB100-598AF350)Find related products by research area.
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Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily... Read full blog post. |
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Further unraveling the role of gamma H2AX in DNA damage response Our genome experiences a moderate amount of DNA damage in our cells on a daily basis. This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry. While normal rates of DNA ... Read full blog post. |
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
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ATM - detecting and responding to DNA damage Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels... Read full blog post. |
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FANCD2 (Fanconi anemia subunit D2 protein) Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama... Read full blog post. |
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53BP1 - a marker for DNA Double Strand Break 53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response... Read full blog post. |
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FANCD2: A big component of the DNA repair crew The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage... Read full blog post. |
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BRCA1 - A Critical Tumor Suppressor Gene in Women Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac... Read full blog post. |
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53BP1 - DNA damage is no fun The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri... Read full blog post. |
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Breast Cancer Infographic Breast cancer is caused by malignant cells developing in breast tissue. It is the most commonly diagnosed cancer in women, but advancements in treatment options have seen the death rate decline since the 1990s. Common warning signs of breast cancer in... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BRCA1 |
![Immunocytochemistry/Immunofluorescence: BRCA2 Antibody [NBP1-88361] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/BRCA2 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88361-img0011.jpg)
![Flow Cytometry: BRCA2 Antibody [NBP1-88361] - DNA damage induced by low-dose radiation and cfDNAox and cfDNAoxR fragments activates reparation of nuclear DNA. (a) (1) Flow cytometry detection of BRCA2 in irradiated (10cGy) and control cells stained with anti-BRCA2 antibodies and secondary FITC-conjugated antibodies. Gate R encircles the fraction of MSCs with elevated values of FL1- BRCA2; (2) distribution of cells with varying BRCA2 contents. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/omcl/2017/9515809/), licensed under a CC-BY licence.](http://images.novusbio.com/images2/BRCA2-Antibody-Flow-Cytometry-NBP1-88361-img0012.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/images2/ATM-Antibody-(2C1)-Western-Blot-NB100-309-img0016.jpg)
![Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/images2/ATM-Antibody-(2C1)-Immunohistochemistry-Paraffin-NB100-309-img0011.jpg)
![Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using ATM antibody (2C1) [NB100-309] by western blot or immunoprecipitation. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/images2/ATM-Antibody-(2C1)-Western-Blot-NB100-309-img0018.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Chk2 Antibody (73C175.1.1)-Western Blot-NB100-56546-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Chk2-Antibody-(73C175.1.1)-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/Chk2-Antibody-(73C175.1.1)-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Western-Blot-NB100-148-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Immunocytochemistry-Immunofluorescence-NB100-148-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.](http://images.novusbio.com/images2/Rad51-Antibody-(14B4)-Immunohistochemistry-Paraffin-NB100-148-img0003.jpg)
![Western Blot: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of PGR expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Western-Blot-H00005241-M08-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Immunocytochemistry-Immunofluorescence-H00005241-M08-img0006.jpg)
![Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml](http://images.novusbio.com/images2/Progesterone-R-NR3C3-Antibody-(4E9)-Immunohistochemistry-Paraffin-H00005241-M08-img0007.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/images2/p21-CIP1-CDKN1A-Antibody-(WA-1-(HJ21))-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of MLH1 on A431 cells lysates using anti-MLH1 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/MLH1 Antibody (SP08-04)-Western Blot-NBP2-67381-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MLH1-Antibody-(SP08-04)-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/MLH1-Antibody-(SP08-04)-Immunohistochemistry-Paraffin-NBP2-67381-img0003.jpg)
![Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.](http://images.novusbio.com/images2/p16INK4a-CDKN2A-Antibody-Western-Blot-NB200-106-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0004.jpg)
![Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0006.jpg)
![Western Blot: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, Hep-G2, K-562, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/BARD1-Antibody-Western-Blot-NB100-319-img0016.jpg)
![Western Blot: BARD1 Antibody [NB100-319] - Detection of Human BARD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat, cells. Antibodies: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/BARD1-Antibody-Western-Blot-NB100-319-img0013.jpg)
![Immunoprecipitation: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BARD1 antibody NB100-319 (lot 2) used for IP at 6 ug/mg lysate. BARD1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BARD1, NB100-319 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/BARD1-Antibody-Immunoprecipitation-NB100-319-img0014.jpg)
![Knockout Validated: Nbs1 Antibody [NB100-143] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Polyclonal [NB100-143] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Nbs1-Antibody-Knockout-Validated-NB100-143-img0015.jpg)
![Western Blot: Nbs1 Antibody [NB100-143] - Western Blot with Nbs1 Antibody [NB100-143]. KSHV LANA recruits MRN (Mre11-Rad50-NBS1) complex. Co-immunoprecipitation of endogenous LANA and MRN proteins in BC3 cells. Cells were lysed using TBS-T buffer and the cell lysate was incubated with benzonase. After centrifugation, supernatant was incubated overnight with anti-LANA or IgG-control beads. The precipitated complexes were analyzed for the presence of endogenous Rad50, Mre11 and NBS1 by SDS-PAGE and immunoblotting. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.ppat.1006335), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Nbs1 Antibody-Western Blot-NB100-143-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - ICC/IF analysis of Nbs1 in HeLa cells with Nbs1 Antibody [NB100-143]. Image from verified customer review.](http://images.novusbio.com/images2/Nbs1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-143-img0011.jpg)
![Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [473] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Western-Blot-NBP2-43728-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 antibody [473] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Immunocytochemistry-Immunofluorescence-NBP2-43728-img0003.jpg)
![Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) 12 % SDS-PAGE COL11A2 antibody [473] dilution: 1:5000](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Western-Blot-NBP2-43728-img0002.jpg)
![Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.](http://images.novusbio.com/images2/53BP1-Antibody-Immunohistochemistry-Frozen-NB100-304-img0015.jpg)
![Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/images2/53BP1-Antibody-Knockout-Validated-NB100-304-img0031.jpg)
![Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.](http://images.novusbio.com/images2/53BP1-Antibody-Western-Blot-NB100-304-img0029.jpg)
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/images2/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/images2/BRIP1-FANCJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31883-img0013.jpg)
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - Paraffin-embedded SAS xenograft; antibody dilution 1:500.](http://images.novusbio.com/images2/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/images2/ATR-Antibody-(2B5)-Western-Blot-NB100-308-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/ATR Antibody (2B5)-Immunocytochemistry-Immunofluorescence-NB100-308-img0001.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/images2/ATR-Antibody-(2B5)-Western-Blot-NB100-308-img0004.jpg)