BRAF35 Antibody - BSA Free

Western Blot: BRAF35 Antibody [NBP2-15608] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: BRAF35 Antibody [NBP2-15608] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Immunoprecipitation: BRAF35 Antibody [NBP2-15608] - 293T whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 ug of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BRAF35 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRAF35 Antibody - BSA Free (NBP2-15608) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BRAF35. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (97%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMG20B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BRAF35 Antibody - BSA Free

• BRAF35Sox-like transcriptional factor
• high-mobility group 20B
• HMG domain-containing protein 2
• HMG domain-containing protein HMGX2
• HMGX2FLJ26127
• HMGXB2member 1-related
• SMARCE1R
• SMARCE1-related protein

Background

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HMG20B
• Uniprot:
  • Q9P0W2(Human)