Boll Antibody

Western Blot: Boll Antibody [H00066037-D01P] - Analysis of BOLL expression in mouse testis.

Immunocytochemistry/Immunofluorescence: Boll Antibody [H00066037-D01P] - Analysis of antibody to BOLL on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: Boll Antibody [H00066037-D01P] - Analysis of BOLL expression in transfected 293T cell line by BOLL polyclonal antibody.Lane 1: BOLL transfected lysate(32.60 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Boll Antibody Summary

• Immunogen: BOLL (NP_932074.1, 1 a.a. - 295 a.a.) full-length human protein. METESGPQTSNQMQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY
• Specificity: BOLL - bol, boule-like (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BOLL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Boll Antibody

• bol, boule-like (Drosophila)
• BOULEbol (Drosophila boule homolog)-like
• protein boule-like

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BOLL
• Entrez:
  • 66037(Human)
• Uniprot:
  • NP_932074.1(Human)