BNP Antibody - Pro form

Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pro‑BNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3604) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro‑BNP at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BNP Antibody - Pro form Summary

• Immunogen: E. coli-derived recombinant human Pro-BNP
  His27-His134
  Accession # P16860
• Specificity: Detects human Pro-BNP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPPB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BNP Antibody - Pro form

• BNF
• BNP
• brain type natriuretic peptide
• Gamma-brain natriuretic peptide
• natriuretic peptide B
• natriuretic peptide precursor B
• natriuretic peptides B
• natriuretic protein
• NPPB

Background

Human BNP (brain natriuretic peptide) is a 3.5 kDa, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12 kDa, 108 amino acid (aa) prohormone with a 76 aa prosegment and a 32 aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27 kDa glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPPB
• Uniprot:
  • P16860()