BNP Antibody (2D11) - Azide and BSA Free

Western Blot: NPPB Antibody (2D11) [H00004879-M01] - Analysis of NPPB expression in transfected 293T cell line by NPPB monoclonal antibody (M01), clone 2D11.Lane 1: NPPB transfected lysate(14.726 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: NPPB Antibody (2D11) [H00004879-M01] - Analysis of NPPB transfected lysate using anti-NPPB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NPPB MaxPab rabbit polyclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clone: 2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BNP Antibody (2D11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BNP (AAH25785, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDPQTAPSRALLLLLFLHLAFLGGRSHPLGSPGSASDSETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPRSPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPPB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BNP Antibody (2D11) - Azide and BSA Free

• BNF
• BNP
• brain type natriuretic peptide
• Gamma-brain natriuretic peptide
• natriuretic peptide B
• natriuretic peptide precursor B
• natriuretic peptides B
• natriuretic protein
• NPPB

Background

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPPB Antibody (H00004879-M01)(1)

Publication using H00004879-M01

• Chiu YW, Chen WP, Su CC et al. The arrhythmogenic effect of self-assembling nanopeptide hydrogel scaffolds on neonatal mouse cardiomyocytes. Nanomedicine. 2014-01-22 [PMID: 24491398]

Bioinformatics

• Gene Symbol: NPPB
• Entrez:
  • 4879(Human)
• OMIM:
  • 600295(Human)
• Uniprot:
  • AAH25785(Human)