BMPR-IB/ALK-6 Antibody (477914) [Unconjugated]

PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR‑IB/ALK‑6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibosy (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

BMPR-IB/ALK-6 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 477914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BMPR-IB/ALK-6 Antibody (477914) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
  Lys14-Arg126
  Accession # O00238
• Specificity: Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMPR1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMPR-IB/ALK-6 Antibody (477914) [Unconjugated]

• activin receptor-like kinase 6
• ALK-6
• ALK6CDw293
• BMP type-1B receptor
• BMPR1B
• BMPR-1B
• BMPRIB
• BMPR-IB
• bone morphogenetic protein receptor type-1B
• bone morphogenetic protein receptor, type IB
• CDw293 antigen
• CDw293
• EC 2.7.11
• EC 2.7.11.30
• serine/threonine receptor kinase

Background

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMPR-IB/ALK-6 Antibody (MAB5051)(5)

Publications using MAB5051

• Nadia Trivieri, Alberto Visioli, Gandino Mencarelli, Maria Grazia Cariglia, Laura Marongiu, Riccardo Pracella et al. Growth factor independence underpins a paroxysmal, aggressive Wnt5aHigh/EphA2Low phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapy Journal of Experimental & Clinical Cancer Research 2022-12-01 [PMID: 35414102]
• Hendrik Boog, Rebecca Medda, Elisabetta Ada Cavalcanti-Adam Single Cell Center of Mass for the Analysis of BMP Receptor Heterodimers Distributions Journal of Imaging 2021-10-20 [PMID: 34821850] (Immunocytochemistry, Human)
• Maëva Chauvin, Véronique Garambois, Sylvie Choblet, Pierre-Emmanuel Colombo, Myriam Chentouf, Laurent Gros et al. Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival International Journal of Oncology 2021-05-17 [PMID: 34013359]
• SV Hegarty, AM Sullivan, GW O'Keeffe Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth Neurosci. Lett, 2017-02-08;643(0):32-37. 2017-02-08 [PMID: 28188849] (ICC, Human)
• Bolander J, Ji W, Geris L et al. The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells. Eur Cell Mater. 1/5/2016 [PMID: 26728496]

Bioinformatics

• Gene Symbol: BMPR1B
• Uniprot:
  • O00238()