BMPR-IB/ALK-6 Antibody (477914) [Allophycocyanin]

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ALK-6 APC-conjugated Monoclonal Antibody (Catalog # FAB5051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 APC-conjugated Monoclonal Antibody (Catalog # FAB5051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 477914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

BMPR-IB/ALK-6 Antibody (477914) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
  Lys14-Arg126
  Accession # O00238
• Specificity: Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMPR1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMPR-IB/ALK-6 Antibody (477914) [Allophycocyanin]

• activin receptor-like kinase 6
• ALK-6
• ALK6CDw293
• BMP type-1B receptor
• BMPR1B
• BMPR-1B
• BMPRIB
• BMPR-IB
• bone morphogenetic protein receptor type-1B
• bone morphogenetic protein receptor, type IB
• CDw293 antigen
• CDw293
• EC 2.7.11
• EC 2.7.11.30
• serine/threonine receptor kinase

Background

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMPR-IB/ALK-6 Antibody (FAB5051A)(3)

Publications using FAB5051A

• V Grotheer, N Skrynecki, L Oezel, J Grassmann, J Windolf, CV Suschek Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence Scientific Reports, 2021-06-07;11(1):11968. 2021-06-07 [PMID: 34099837] (Flow Cytometry, Human)
• P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal Cell Death Dis, 2018-09-11;9(9):927. 2018-09-11 [PMID: 30206237] (Flow Cytometry, Human)
• GB Ferguson, B Van Handel, M Bay, P Fiziev, T Org, S Lee, R Shkhyan, NW Banks, M Scheinberg, L Wu, B Saitta, J Elphingsto, AN Larson, SM Riester, AD Pyle, NM Bernthal, HK Mikkola, J Ernst, AJ van Wijnen, M Bonaguidi, D Evseenko Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes Nat Commun, 2018-09-07;9(1):3634. 2018-09-07 [PMID: 30194383] (Cell Selection, Human)

Bioinformatics

• Gene Symbol: BMPR1B
• Uniprot:
  • O00238()