BMP‑6 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human BMP‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF507) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Recombinant Human BMP‑6 (Catalog # 507‑BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by ...read more
E. coli-derived recombinant human BMP‑6 Gln382-His513 Accession # P22004
Detects human BMP‑6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) BMP-7 is observed and less than 1% cross-reactivity with rhBMP-2, rhBMP-4, rhBMP-5mature, rhBMP-8A, and recombinant mouse BMP-6 is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BMP-6 Antibody [Unconjugated]
bone morphogenetic protein 6
vegetal related growth factor (TGFB-related)
vegetal-related (TGFB related) cytokine
Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7 and -8 are in the same subgroup.
Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
Reddi, A.H. (1998) Nature Biotechnology 16:247.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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