Human BMP-4 Recombinant Protein

Product Discontinued

Human BMP-4 Recombinant Protein Summary

• Description: A homodimeric protein consisting of two 116 residues glycosylated polypeptide chains and corresponding to amino acids Ser293 - Arg408 of BMP4.
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: The ED50, as determined by inducing alkaline phosphatase production using murine ATDC5 cells, is less than 25 ng/ml.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: BMP4
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rHuBMP-4 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 37-41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute with 4 mM HCl to a final concentration of 0.05 - 0.2 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human BMP-4 Recombinant Protein

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature BMP-4 forms homodimers and heterodimers with BMP-7. BMP-4/BMP-7 heterodimers show greater potency at osteogenic differentiation compared to BMP-4 homodimers. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BMP4