BMI-1 Antibody


Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Reactivity Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, ZeSpecies Glossary
Applications WB, ICC/IF
1.0 mg/ml

BMI-1 Antibody Summary

A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]
Predicted Species
Rat (92%), Chicken (92%), Primate (92%), Bovine (92%), Rabbit (92%), Zebrafish (92%), Feline (92%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This Bmi1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.
BMI-1 Overexpression Lysate
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
BMI-1 Peptide (NB110-40823PEP)
Read Publication using NB110-40823.

Reactivity Notes

Human and mouse. Immunogen displays the following percentage of sequence identity for non-tested species: primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein (all 92%).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for BMI-1 Antibody

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4


The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMI-1 Antibody (NB110-40823)(1)

We have publications tested in 1 confirmed species: Mouse.

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Product General Protocols

View specific protocols for BMI-1 Antibody (NB110-40823): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BMI-1 Antibody (NB110-40823) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40823AF350
Alexa Fluor 405 NB110-40823AF405
Alexa Fluor 488 NB110-40823AF488
Alexa Fluor 532 NB110-40823AF532
Alexa Fluor 594 NB110-40823AF594
Alexa Fluor 647 NB110-40823AF647
Alexa Fluor 700 NB110-40823AF700
Alexa Fluor 750 NB110-40823AF750
Biotin NB110-40823B
DyLight 350 NB110-40823UV
DyLight 405 NB110-40823V
DyLight 488 NB110-40823G
DyLight 550 NB110-40823R
DyLight 594 NB110-40823DL594
DyLight 650 NB110-40823C
DyLight 680 NB110-40823FR
DyLight 755 NB110-40823IR
FITC NB110-40823F
HRP NB110-40823H
Janelia Fluor 549 NB110-40823JF549
Janelia Fluor 646 NB110-40823JF646

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Gene Symbol BMI1