BMI-1 Antibody

Images

 
Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

BMI-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]
Localization
Nuclear
Predicted Species
Rat (92%), Chicken (92%), Primate (92%), Bovine (92%), Rabbit (92%), Zebrafish (92%), Feline (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BMI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This Bmi1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.
Positive Control
BMI-1 Lysate (NBL1-07997)
Skeletal Muscle Lysate (NB820-59253)
Skeletal Muscle Lysate (NB820-59668)
Control Peptide
BMI-1 Peptide (NB110-40823PEP)
Publications
Read Publication using NB110-40823.

Reactivity Notes

Human and mouse. Immunogen sequence has 92% homology to primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BMI-1 Antibody

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-33748
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-29965
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP1-88856
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
21650002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
H00006045-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-37370
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-317
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB120-16518
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB110-40823
Species: Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, Ze
Applications: WB, ICC/IF

Publications for BMI-1 Antibody (NB110-40823)(1)

We have publications tested in 1 confirmed species: Mouse.


Filter By Application
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for BMI-1 Antibody (NB110-40823) (0)

There are no reviews for BMI-1 Antibody (NB110-40823). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for BMI-1 Antibody (NB110-40823): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BMI-1 Antibody (NB110-40823) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-40823AF405
Alexa Fluor 488 Labeled NB110-40823AF488
Alexa Fluor 647 Labeled NB110-40823AF647
Alexa Fluor 700 Labeled NB110-40823AF700
Biotin Labeled NB110-40823B
DyLight 405 Labeled NB110-40823V
DyLight 488 Labeled NB110-40823G
DyLight 550 Labeled NB110-40823R
DyLight 650 Labeled NB110-40823C
FITC Labeled NB110-40823F
HRP Labeled NB110-40823H

Additional BMI-1 Products

Bioinformatics Tool for BMI-1 Antibody (NB110-40823)

Discover related pathways, diseases and genes to BMI-1 Antibody (NB110-40823). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BMI-1 Antibody (NB110-40823)

Discover more about diseases related to BMI-1 Antibody (NB110-40823).
 

Pathways for BMI-1 Antibody (NB110-40823)

View related products by pathway.

PTMs for BMI-1 Antibody (NB110-40823)

Learn more about PTMs related to BMI-1 Antibody (NB110-40823).
 

Research Areas for BMI-1 Antibody (NB110-40823)

Find related products by research area.

Blogs on BMI-1

There are no specific blogs for BMI-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BMI-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol BMI1
Entrez