Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Immunogen affinity purified
This Bmi1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.
Human and mouse. Immunogen sequence has 92% homology to primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein.
Alternate Names for BMI-1 Antibody
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
B lymphoma Mo-MLV insertion region 1 homolog
BMI1 polycomb ring finger oncogene
murine leukemia viral (bmi-1) oncogene homolog
polycomb complex protein BMI-1
polycomb group protein Bmi1
polycomb group ring finger 4
RING finger protein 51
RNF51Polycomb group RING finger protein 4
The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.