BMI-1 Antibody

Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle

Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

BMI-1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]
• Localization: Nuclear
• Predicted Species: Rat (92%), Chicken (92%), Primate (92%), Bovine (92%), Rabbit (92%), Zebrafish (92%), Feline (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
• Application Notes: This Bmi1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.
• Control:

  BMI-1 Overexpression Lysate
  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  BMI-1 Peptide (NB110-40823PEP)

Reactivity Notes

Human and mouse. Immunogen sequence has 92% homology to primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BMI-1 Antibody

• B lymphoma Mo-MLV insertion region 1 homolog (mouse)
• B lymphoma Mo-MLV insertion region 1 homolog
• BMI1 polycomb ring finger oncogene
• BMI1
• BMI-1
• FLVI2/BMI1
• MGC12685
• murine leukemia viral (bmi-1) oncogene homolog
• PCGF4
• polycomb complex protein BMI-1
• polycomb group protein Bmi1
• polycomb group ring finger 4
• RING finger protein 51
• RNF51
• RNF51Polycomb group RING finger protein 4

Background

The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMI-1 Antibody (NB110-40823)(1)

Publication using NB110-40823

• Kang X, Qi Y, Zuo Y et al. SUMO-specific protease 2 is essential for suppression of polycomb group protein-mediated gene silencing during embryonic development. Mol Cell 2010 Apr 23 [PMID: 20417598] (Mouse)

Bioinformatics

• Gene Symbol: BMI1