BMI-1 Antibody


Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
1.0 mg/ml

BMI-1 Antibody Summary

A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]
Predicted Species
Rat (92%), Zebrafish (92%), Bovine (92%), Primate (92%), Rabbit (92%), Chicken (92%), Feline (92%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This Bmi1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.
Control Peptide
BMI-1 Peptide (NB110-40823PEP)
Read Publication using NB110-40823.

Reactivity Notes

Human and mouse. Immunogen sequence has 92% homology to primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein.

Alternate Names for BMI-1 Antibody

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4


The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMI-1 Antibody (NB110-40823)(1)

We have publications tested in 1 confirmed species: Mouse.

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Product General Protocols

View specific protocols for BMI-1 Antibody (NB110-40823): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-40823AF405
Alexa Fluor (R) 488 Labeled NB110-40823AF488
Alexa Fluor (R) 647 Labeled NB110-40823AF647
Alexa Fluor (R) 700 Labeled NB110-40823AF700
Biotin Labeled NB110-40823B
DyLight 405 Labeled NB110-40823V
DyLight 488 Labeled NB110-40823G
DyLight 550 Labeled NB110-40823R
DyLight 650 Labeled NB110-40823C
FITC Labeled NB110-40823F
HRP Labeled NB110-40823H

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Blogs on BMI-1

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Contact Information

Product PDFs


Gene Symbol BMI1

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