BLZF1 Antibody

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Western Blot: BLZF1 Antibody [NBP2-15596] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody diluted at 1:5000. ...read more
Immunocytochemistry/ Immunofluorescence: BLZF1 Antibody [NBP2-15596] - BLZF1 antibody detects BLZF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at ...read more
Western Blot: BLZF1 Antibody [NBP2-15596] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: BLZF1 Antibody [NBP2-15596] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BLZF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human BLZF1. The exact sequence is proprietary.
Localization
Golgi apparatus lumen; Isoform 1: Nucleus; Isoform 2: Cytoplasm
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BLZF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Rat (84%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for BLZF1 Antibody

  • basic leucine zipper nuclear factor 1JEM-1s
  • cytoplasmic protein
  • GOLGIN-45
  • JEM1
  • JEM-1MGC22497
  • JEM-1short protein
  • p45 basic leucine-zipper nuclear factor

Background

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BLZF1
Uniprot