BLNK Antibody

Western Blot: BLNK Antibody [NB100-804] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: BLNK Antibody [NB100-804] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: BLNK Antibody [NB100-804] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BLNK Antibody Summary

• Description: Novus Biologicals Goat BLNK Antibody (NB100-804) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-BLNK Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KDSTRLKYAVKVS corresponding to C-Terminus according to NP_037446.1, NP_001107566.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_037446.1; NP_001107566.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BLNK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 70 kDa band observed in Daudi lysates (calculated MW of 50.5 kDa band according to NP_037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50 kDa band is observed.
• Control:

  BLNK Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BLNK Antibody

• AGM4
• B cell linker protein
• BASH
• B-cell adapter containing a SH2 domain protein
• B-cell adapter containing a Src homology 2 domain protein
• B-cell linker protein
• B-cell linker
• BLNK
• BLNK-s
• Cytoplasmic adapter protein
• Ly57
• MGC111051
• SLP65
• SLP-65BLNK-S
• SLP65Ly57
• Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLNK Antibody (NB100-804)(2)

Publications using NB100-804

• Cawley E, Tippler M, Coupland NJ et al. Dopamine and light: effects on facial emotion recognition. Journal of psychopharmacology (Oxford, England) 2018-05-17 [PMID: 28633582]
• Fu C, Turck CW, Kurosaki T, Chan AC. BLNK: a central linker protein in B cell activation. Immunity 1998-07-01 [PMID: 9697839]

Bioinformatics

• Gene Symbol: BLNK
• Entrez:
  • 29760(Human)
  • 17060(Mouse)
• Uniprot:
  • NP_037446.(Human)