BLAME/SLAMF8 Antibody

Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with NBP2-26110 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26110 (1 ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BLAME/SLAMF8 Antibody Summary

• Immunogen: Peptide with sequence C-TLYHSRFLGRAQ corresponding to internal region according to NP_064510.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLAMF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100 - 1:2000
• Application Notes: Western blot: In transfected HEK293 transiently expressing Human SLAMF8 (myc and DYKDDDDK tagged), a band of approx. 45kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
  IHC: Paraffin embedded Human Small Intestine.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BLAME/SLAMF8 Antibody

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLAME/SLAMF8 Antibody (NBP2-26110)(1)

Publication using NBP2-26110

• Davila S, Froeling FE, Tan A et al. New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun 2010-04-01 [PMID: 20237496]

Bioinformatics

• Gene Symbol: SLAMF8
• Entrez:
  • 56833(Human)
  • 74748(Mouse)
  • 289237(Rat)