BLAME/SLAMF8 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BLAME/SLAMF8 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BLAME/SLAMF8
  Val21-Asp231
  Accession # Q9D3G2
• Specificity: Detects mouse BLAME/SLAMF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human BLAME is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLAME/SLAMF8 Antibody

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Background

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of the Ig superfamily cell receptors. The 258 amino acid (aa) mature mouse BLAME contains a 211 aa extracellular domain (ECD) with an Ig V- and a C2-like domain, and a 26 aa cytoplasmic tail that lacks signaling motifs. BLAME is expressed by activated monocytes and dendritic cells. Although the function of BLAME is not known, it is likely involved in homotypic binding or heterotypic interactions with other CD2 subset members. Over the ECD region used as immunogen, mouse BLAME shares 95% and 78% aa identity with rat and human BLAME, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLAME/SLAMF8 Antibody (AF4156)(1)

Publication using AF4156

• Wang G, van Driel B, Liao G, O'Keeffe M, Halibozek P, Flipse J, Yigit B, Azcutia V, Luscinskas F, Wang N, Terhorst C Migration of myeloid cells during inflammation is differentially regulated by the cell surface receptors Slamf1 and Slamf8. PLoS ONE, 2015-03-23;10(3):e0121968. 2015-03-23 [PMID: 25799045] (Western Blot, Mouse)

Bioinformatics

• Uniprot:
  • Q9D3G2()