BID Antibody (91508) [Unconjugated]

Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western blot shows lysates of SVEC4‑10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 91508
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

BID Antibody (91508) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse BID
  Met1-Asp195
  Accession # P70444
• Specificity: Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: BID

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BID Antibody (91508) [Unconjugated]

• apoptic death agonist
• BH3 interacting domain death agonist
• BH3-interacting domain death agonist
• BID isoform ES(1b)
• BID isoform L(2)
• BID isoform Si6
• BID
• desmocollin type 4
• FP497
• Human BID coding sequence
• MGC15319
• MGC42355
• p22 BID

Background

BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is cytosolic in healthy cells but upon delivery of an apoptotic signal, BID is cleaved by Caspase-8. The cleaved form is translocated to the mitochondria outer membrane where it binds to BAK and the resulting complex causes altered mitochondrial membrane permeability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BID Antibody (MAB860)(6)

Publications using MAB860

• E Kip, F Nazé, V Suin, T Vanden Ber, A Francart, S Lamoral, P Vandenabee, R Beyaert, S Van Gucht, M Kalai Impact of caspase-1/11, -3, -7, or IL-1?/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease Cell Death Discov, 2017;3(0):17012. 2017 [PMID: 28280602] (Western Blot, Mouse)
• Zhu, Wenhan, Tao, Lili, Quick, Marsha L, Joyce, Johanna, Qu, Jie-Ming, Luo, Zhao-Qin Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection. PLoS Pathog, 2015;11(3):e1004704. 2015 [PMID: 25738962] (Western Blot, Mouse)
• Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014;21(9):1442-50. 2014 [PMID: 24902901] (Western Blot, Mouse)
• Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor. J. Neurochem., 2010;115(1):92-101. 2010 [PMID: 20646170] (Western Blot, Mouse)
• Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis. J. Neurochem., 2007;102(4):1206-19. 2007 [PMID: 17488272] (Western Blot, Mouse)
• Caruso JA, Reiners JJ Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop. Apoptosis, 2006;11(11):1877-85. 2006 [PMID: 17013759] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BID
• Uniprot:
  • P70444()