BID Antibody (91508) [Unconjugated]

Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vector control S. Typhimurium does not activate apoptotic backup pathways in vitro.(A–E) Bone marrow-derived macrophages (BMMs) were infected with indicated SPI2-induced S. Typhimurium strains. (A) Western blot analysis of whole cell lysates at 4 hpi. Results from one experiment. (B) Lactate dehydrogenase (LDH) release at 1–8 hpi. Results representative of three independent experiments. Data are represented as mean ± SD of three technical replicates. Data performed at the same time as Figure 1E, graphed separately for visualization. (C) LDH release at 1–8 hpi. Results from one experiment. Data are represented as mean ± SD of three technical replicates. (D–E) Immunofluorescence and brightfield at 4 hpi. Cells were stained with PI, cleaved caspase-3/7, and Hoechst. Representative image from two (brightfield, PI) or one (cleaved caspase-3/7) independent experiments. Data performed at the same time as Figure 1F. (D) 60 x magnification, scale bar 20 µm. (E) 20 x stitched image, scale bar 500 µm.Figure 1—figure supplement 1—source data 1.Western blot images for Figure 1—figure supplement 1A.Figure 1—figure supplement 1—source data 2.Data for Figure 1—figure supplement 1B–C.Western blot images for Figure 1—figure supplement 1A.Data for Figure 1—figure supplement 1B–C. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38055781), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 91508
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

BID Antibody (91508) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse BID
  Met1-Asp195
  Accession # P70444
• Specificity: Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: BID
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BID Antibody (91508) [Unconjugated]

• apoptic death agonist
• BH3 interacting domain death agonist
• BH3-interacting domain death agonist
• BID isoform ES(1b)
• BID isoform L(2)
• BID isoform Si6
• BID
• desmocollin type 4
• FP497
• Human BID coding sequence
• MGC15319
• MGC42355
• p22 BID
• tbid

Background

BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is cytosolic in healthy cells but upon delivery of an apoptotic signal, BID is cleaved by Caspase-8. The cleaved form is translocated to the mitochondria outer membrane where it binds to BAK and the resulting complex causes altered mitochondrial membrane permeability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BID Antibody (MAB860)(16)

Publications using MAB860

• Abele, TJ;Billman, ZP;Li, L;Harvest, CK;Bryan, AK;Magalski, GR;Lopez, JP;Larson, HN;Yin, XM;Miao, EA; Apoptotic signaling clears engineered Salmonella in an organ-specific manner bioRxiv : the preprint server for biology 2023-05-06 [PMID: 37205464] (Western Blot, Mouse, Transgenic Mouse)
• K Ning, W Zou, P Xu, F Cheng, EY Zhang, A Zhang-Chen, S Kleiboeker, J Qiu Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors Science Advances, 2023-01-11;9(2):eade0869. 1/11/2023 [PMID: 36630517]
• Schleicher EM, Dhoonmoon A, Jackson LM et al. The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells Oncogenesis 6/18/2022 [PMID: 35717336]
• Vianello C, Cocetta V, Catanzaro D et al. Cisplatin resistance can be curtailed by blunting Bnip3-mediated mitochondrial autophagy Cell death & disease 4/22/2022 [PMID: 35459212]
• Shashi Bala, Charles D. Calenda, Donna Catalano, Mrigya Babuta, Karen Kodys, Imad A. Nasser et al. Deficiency of miR‐208a Exacerbates CCl4‐Induced Acute Liver Injury in Mice by Activating Cell Death Pathways Hepatology Communications 2020-10-01 [PMID: 33024918]
• A Markotic, D Flegar, D Grcevic, A Sucur, H Lalic, P Turcic, N Kovacic, N Lukac, D Pravdic, K Vukojevic, I Cavar, T Kelava LPS-induced inflammation desensitizes hepatocytes to Fas-induced apoptosis through Stat3 activation-The effect can be reversed by ruxolitinib J. Cell. Mol. Med., 2020-02-05;0(0):. 2020-02-05 [PMID: 32022429] (Western Blot, Mouse)
• Aina He, Wenqian Fang, Kun Zhao, Yajun Wang, Jie Li, Chongzhe Yang et al. Mast cell-deficiency protects mice from streptozotocin-induced diabetic cardiomyopathy Translational Research 2019-06-01 [PMID: 30738862] (Western Blot, Mouse)
• Yunzhe Wang, Cong-Lin Liu, Wenqian Fang, Xian Zhang, Chongzhe Yang, Jie Li et al. Deficiency of mouse mast cell protease 4 mitigates cardiac dysfunctions in mice after myocardium infarction Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2019-06-01 [PMID: 30639224]
• E Kip, F Nazé, V Suin, T Vanden Ber, A Francart, S Lamoral, P Vandenabee, R Beyaert, S Van Gucht, M Kalai Impact of caspase-1/11, -3, -7, or IL-1?/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease Cell Death Discov, 2017-03-06;3(0):17012. 2017-03-06 [PMID: 28280602] (Western Blot, Mouse)
• J Barroso-González, S Auclair, S Luan, L Thomas, K M Atkins, J E Aslan et al. PACS-2 mediates the ATM and NF-kappa B-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage Cell Death & Differentiation 2016-09-01 [PMID: 26943323]
• Zhu , Wenhan, Tao , Lili, Quick , Marsha L, Joyce , Johanna, Qu , Jie-Ming, Luo , Zhao-Qin Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection. PLoS Pathog, 2015-03-04;11(3):e1004704. 2015-03-04 [PMID: 25738962] (Western Blot, Mouse)
• Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014-06-06;21(9):1442-50. 2014-06-06 [PMID: 24902901] (Western Blot, Mouse)
• Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor. J. Neurochem., 2010-07-30;115(1):92-101. 2010-07-30 [PMID: 20646170] (IHC-Fr, Western Blot, Mouse)
• Chun-Shiang Chung, Fabienne Venet, Yaping Chen, Leslie N. Jones, Douglas C. Wilson, Carol A. Ayala et al. DEFICIENCY OF BID PROTEIN REDUCES SEPSIS-INDUCED APOPTOSIS AND INFLAMMATION, WHILE IMPROVING SEPTIC SURVIVAL Shock 2010-08-01 [PMID: 20023601]
• Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis. J. Neurochem., 2007-05-04;102(4):1206-19. 2007-05-04 [PMID: 17488272] (Western Blot, Mouse)
• Caruso JA, Reiners JJ Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop. Apoptosis, 2006-11-01;11(11):1877-85. 2006-11-01 [PMID: 17013759] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BID
• Uniprot:
  • P70444()