Recombinant Human Betacellulin/BTC Protein

SDS-Page: Human Betacellulin/BTC Protein [NBP2-34919]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Betacellulin/BTC Protein Summary

• Description: Recombinant human Betacellulin is a 9.0 kDa monomeric protein, corresponding to 80 amino residues, which comprises the mature EGF homologous portion of Betacellulin/BTC Source: E. coli

  Uniprot ID: P35070

  Amino Acid Sequence: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BTC
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Betacellulin/BTC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 9.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Betacellulin/BTC Protein

• Betacellulin
• BTC
• probetacellulin

Background

Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine Betacellulin is a heparin binding protein containing 80 amino acids residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BTC
• Uniprot:
  • P35070()
  • P35070()
  • P35070()