beta Tubulin Antibody - BSA Free

Western Blot: beta Tubulin Antibody [NBP2-54728] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-54728] - Staining of human lymph node shows cytoplasmic positivity in germinal center.

Western Blot: beta Tubulin Antibody [NBP2-54728] - Analysis in human cell line U-138MG.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

beta Tubulin Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a Recombinant Protein corresponding to amino acids: GTGSGMGTLLISKIREEYPDRIINTFS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  beta Tubulin Recombinant Protein Antigen (NBP2-54728PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for beta Tubulin Antibody - BSA Free

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB