beta Tubulin Antibody

Images

 
Western Blot: beta Tubulin Antibody [NBP2-15584] - ample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NBP2-15584] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-15584] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.
Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: NIH-3T3, B: JC, C: BCL-1, D: C2C12, E: Raw264. 7 10% SDS PAGE NBP2-15584 diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml

Order Details

beta Tubulin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for beta Tubulin Antibody

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NBP2-15584). (Showing 1 - 1 of 1 FAQ).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.

Secondary Antibodies

 

Isotype Controls

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Blogs on beta Tubulin

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TUBB

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