beta Tubulin Antibody (4E9G8)

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of C6 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of NIH/3T3 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of various lysates using beta -Tubulin Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of lysates from 293F cells, using beta -Tubulin Rabbit mAb at 1:1000-1:30000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (4E9G8) [NBP3-15439] -Analysis of paraffin-embedded Rat kidney tissue using beta -Tubulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Western Blot: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Western blot analysis of various lysates using beta Tubulin Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunofluorescence analysis of U2OS cells using beta Tubulin Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug beta Tubulin antibody . Western blot was performed from the immunoprecipitate using beta Tubulin antibody at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 4E9G8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

beta Tubulin Antibody (4E9G8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human beta Tubulin (P07437). LRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA ,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:5000 - 1:10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for beta Tubulin Antibody (4E9G8)

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB