beta-TrCP1/BTRC Antibody (763524)

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -TrCP1/BTRC at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

beta -TrCP1/BTRC was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for beta -TrCP1/BTRC at approximately 84 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: 763524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

beta-TrCP1/BTRC Antibody (763524) Summary

• Immunogen: E. coli-derived recombinant human beta -TrCP1 isoform 2
  Met1-Leu120
  Accession # Q9Y297-2
• Specificity: Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BTRC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-TrCP1/BTRC Antibody (763524)

• beta-transducin repeat containing
• betaTrCP
• BETA-TRCP
• betaTrCP1
• beta-TrCP1
• BTRC
• BTRCP
• bTrCP1
• E3RSIkappaB
• F-box and WD repeats protein beta-TrCP
• F-box/WD repeat-containing protein 1A
• FBW1A
• FBXW1
• FBXW1A
• FBXW1ABTRCP
• FWD1
• MGC4643
• pIkappaBalpha-E3 receptor subunit

Background

beta -TrCP1, also called BTRC ( beta -transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta -TrCP1 can form homodimers and heterodimers with beta -TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta -TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta -TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta -TrCP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTRC
• Uniprot:
  • Q9Y297-2()