beta-TrCP1/BTRC Antibody (763524)

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human beta -TrCP1/BTRC Monoclonal Antibody ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for beta -TrCP1/BTRC at approximately 84 kDa (as indicated) using 10 µg/mL ...read more
beta ‑TrCP1/BTRC was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human beta ‑TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
763524
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

beta-TrCP1/BTRC Antibody (763524) Summary

Immunogen
E. coli-derived recombinant human beta -TrCP1 isoform 2
Met1-Leu120
Accession # Q9Y297-2
Specificity
Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BTRC
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-TrCP1/BTRC Antibody (763524)

  • beta-transducin repeat containing
  • betaTrCP
  • BETA-TRCP
  • betaTrCP1
  • beta-TrCP1
  • BTRC
  • BTRCP
  • bTrCP1
  • E3RSIkappaB
  • F-box and WD repeats protein beta-TrCP
  • F-box/WD repeat-containing protein 1A
  • FBW1A
  • FBXW1
  • FBXW1A
  • FBXW1ABTRCP
  • FWD1
  • MGC4643
  • pIkappaBalpha-E3 receptor subunit

Background

beta -TrCP1, also called BTRC ( beta -transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta -TrCP1 can form homodimers and heterodimers with beta -TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta -TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta -TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta -TrCP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31363
Species: Hu
Applications: WB, ICC/IF
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP2-75458
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-37539
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-16454
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF

Publications for beta-TrCP1/BTRC Antibody (MAB7675) (0)

There are no publications for beta-TrCP1/BTRC Antibody (MAB7675).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta-TrCP1/BTRC Antibody (MAB7675) (0)

There are no reviews for beta-TrCP1/BTRC Antibody (MAB7675). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-TrCP1/BTRC Antibody (MAB7675) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for beta-TrCP1/BTRC Antibody (MAB7675)

Discover related pathways, diseases and genes to beta-TrCP1/BTRC Antibody (MAB7675). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-TrCP1/BTRC Antibody (MAB7675)

Discover more about diseases related to beta-TrCP1/BTRC Antibody (MAB7675).
 

Pathways for beta-TrCP1/BTRC Antibody (MAB7675)

View related products by pathway.

PTMs for beta-TrCP1/BTRC Antibody (MAB7675)

Learn more about PTMs related to beta-TrCP1/BTRC Antibody (MAB7675).

Blogs on beta-TrCP1/BTRC

There are no specific blogs for beta-TrCP1/BTRC, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta-TrCP1/BTRC Antibody (763524) and receive a gift card or discount.

Bioinformatics

Gene Symbol BTRC
Uniprot